Rhizoplane microbiota of superior wheat varieties possess enhanced plant growth-promoting abilities

doi:10.1007/s11515-016-1426-y

Front. Biol.

2016, Vol. 11

Issue (6) : 481-487 https://doi.org/10.1007/s11515-016-1426-y

RESEARCH ARTICLE

Rhizoplane microbiota of superior wheat varieties possess enhanced plant growth-promoting abilities

Ayesha Siddiqa¹,Yasir Rehman¹(

),Shahida Hasnain^1,²

¹. Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan
². The Women University Multan, Multan, Pakistan

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Abstract

BACKGROUND: Microbes affect the growth of plants. In this study, the diversity and plant growth-supporting activities of wheat rhizospheric bacteria were examined.

METHODS: Sampling was performed thrice at different phases of plant growth. Microbes associated with the rhizoplane of three wheat varieties (Seher, Lasani, and Faisalabad) were cultured and assessed for their plant growth-promoting abilities based on auxin production, hydrogen cyanide production, phosphate solubilization, and nitrogen fixation.

RESULTS: Bacterial load (CFU/mL) declined, and the succession of bacterial diversity occurred as the plants aged. Most auxin-producing bacteria and the highest concentrations of auxin (77 µg/mL) were observed during the second sampling point at the tillering stage. The Seher variety harbored the most auxin-producing as well as phosphate-solubilizing bacteria. Most of the bacteria belonged to Bacillus and Pseudomonas. Planomicrobium, Serratia, Rhizobium, Brevundimonas, Stenotrophomonas, and Exiguobacterium sp. were also found.

CONCLUSIONS: These results suggest that the rhizoplane microbiota associated with higher-yield plant varieties have better plant growth-promoting abilities as compared to the microbiota associated with lower-yield plant varieties.

Keywords wheat microbiota rhizoplane auxin phosphate solubilization

Corresponding Author(s): Yasir Rehman

Online First Date: 01 December 2016 Issue Date: 26 December 2016

Cite this article:

Ayesha Siddiqa,Yasir Rehman,Shahida Hasnain. Rhizoplane microbiota of superior wheat varieties possess enhanced plant growth-promoting abilities[J]. Front. Biol., 2016, 11(6): 481-487.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-016-1426-y
https://academic.hep.com.cn/fib/EN/Y2016/V11/I6/481

Fig.1 Auxin production by bacterial isolates of the rhizoplanes of three wheat varieties (Seher, Faisalabad, and Lasani) at the first sampling point. Most auxin producers were found in the rhizoplane of the Faisalabad variety. The highest auxin concentration was observed for LAR1-4 from the Lasani variety.

(SHR= Seher, FDR= Faisalabad, LAR= Lasani)

Fig.2 Auxin production by bacterial isolates of the rhizoplanes of three wheat varieties (Seher, Faisalabad, and Lasani) at the second sampling point. Most auxin producers were found in the rhizoplane of the Seher variety at the second sampling point, but highest auxin concentration was observed for FDR2-4 from the Faisalabad variety.

(SHR= Seher, FDR= Faisalabad, LAR= Lasani)

Fig.3 Auxin production by bacterial isolates of the rhizoplanes of three wheat varieties (Seher, Faisalabad, and Lasani) at the third sampling point. Most auxin producers were again detected in the rhizoplane of the Seher variety. The highest auxin concentration was observed for SHR3-5.

(SHR= Seher, FDR= Faisalabad, LAR= Lasani)

Fig.4 Neighbor-joining phylogenetic tree of the 16S rRNA gene sequences of the selected bacterial isolates. The tree was constructed by aligning the sequences and their nearest homologous sequences from NCBI using Mega 5. One hundred bootstrap replicates were used to measure reliability. Most of the bacterial isolates were identified as Firmicutes.

(SHR= Seher, FDR= Faisalabad, LAR= Lasani)

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