Complete mitochondrial genome of <i>Ampittia dioscorides</i> (Lepidoptera: Hesperiidae) and its phylogenetic analysis

doi:10.1007/s11515-016-1434-y

Front. Biol.

2017, Vol. 12

Issue (1) : 71-81 https://doi.org/10.1007/s11515-016-1434-y

RESEARCH ARTICLE

Complete mitochondrial genome of Ampittia dioscorides (Lepidoptera: Hesperiidae) and its phylogenetic analysis

Xin-Min Qin(

),Xiao-Wen Yang,Li-Xia Hou,Hui-Min Li

Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004, China

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Abstract

The complete mitochondrial genome of Ampittia dioscorides (Lepidoptera: Hesperiidae) was determined. The sequenced genome is a circular molecule of 15313 bp, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an A+ T-rich region. The gene arrangements and transcribing directions are identical to those in most of the reported lepidopteran mitogenomes. The base composition of the whole genome and genes or regions are also similar to those in other lepidopteran species. All the PCGs are initiated by typical ATN codons; the exception being COI, which begins with a CGA codon. Eight genes (ND2, ATPase8, ATPase6, COIII, ND5, ND4L, ND6, and Cytb) end with a TAA stop codon, and two genes (ND1 and ND3) end with TAG. The remaining three genes (COI and COII, which end with TA-, and ND4, which ends with T-) have incomplete stop codons. All tRNAs have the typical clover-leaf structure of mitochondrial tRNAs, with the exception of tRNA^Ser(AGY). On the basis of the concatenated nucleotide and amino acid sequences of the 13 PCGs and wingless gene of 22 butterfly species, maximum parsimony (MP) and Bayesian inference (BI) trees were constructed, respectively. Both MP and BI trees had the same topological structure: ((((Nymphalidae+ Danaidae) + Lycaenidae) + Pieridae) + Papilionidae) + Hesperiidae). The results provide support for Hesperiidae as a superfamily-level taxon.

Keywords Ampittia dioscorides mitochondrial genome phylogeny

Corresponding Author(s): Xin-Min Qin

Online First Date: 13 January 2017 Issue Date: 28 February 2017

Cite this article:

Xin-Min Qin,Xiao-Wen Yang,Li-Xia Hou, et al. Complete mitochondrial genome of Ampittia dioscorides (Lepidoptera: Hesperiidae) and its phylogenetic analysis[J]. Front. Biol., 2017, 12(1): 71-81.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-016-1434-y
https://academic.hep.com.cn/fib/EN/Y2017/V12/I1/71

Tab.1 List of primers used to amplify and sequence the mitogenome of Ampittia dioscorides

Tab.2 Related information on the complete mitogenome used in the phylogenetic analyses

Tab.3 Summary of the mitogenome of Ampittia dioscorides

Fig.1 Circular map of the mitochondrial genome of Ampittia dioscorides

Fig.2 Predicted secondary clover-leaf structure for the tRNA genes of Ampittia dioscorides.

Fig.3 Maximum parsimony phylogenetic tree based on the concatenated amino acid sequences of 13 protein coding genes+ wingless genes.

Fig.4 Bayesian inference phylogenetic tree based on the concatenated amino acid sequences of 13 protein coding genes+ wingless genes.

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