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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

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Front. Med.    2016, Vol. 10 Issue (1) : 85-90    https://doi.org/10.1007/s11684-016-0435-1
RESEARCH ARTICLE
Coronary leukocyte activation in relation to progression of coronary artery disease
Marijke A. de Vries1,*(),Arash Alipour1,Erwin Birnie2,Andrew Westzaan1,Selvetta van Santen1,Ellen van der Zwan3,Anho H. Liem4,Noëlle van der Meulen1,Manuel Castro Cabezas1
1. Department of Internal Medicine, Centre for Diabetes and Vascular Medicine
2. Department of Statistics and Education
3. Department of Clinical Chemistry
4. Department of Cardiology, Sint Franciscus Gasthuis, Rotterdam, P.O. Box 10900, 3004 BA, The Netherlands
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Abstract

Leukocyte activation has been linked to atherogenesis, but there is little in vivo evidence for its role in the progression of atherosclerosis. We evaluated the predictive value for progression of coronary artery disease (CAD) of leukocyte activation markers in the coronary circulation. Monocyte and neutrophil CD11b, neutrophil CD66b expression and intracellular neutrophil myeloperoxidase (MPO) in the coronary arteries were determined by flow cytometry in patients undergoing coronary angiography. The primary outcome included fatal and nonfatal myocardial infarction or arterial vascular intervention due to unstable angina pectoris. In total 99 subjects who were included, 70 had CAD at inclusion (26 patients had single-vessel disease, 18 patients had two-vessel disease and 26 patients had three-vessel disease). The median follow-up duration was 2242 days (interquartile range: 2142–2358). During follow-up, 13 patients (13%) developed progression of CAD. Monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO measured in blood obtained from the coronary arteries were not associated with the progression of CAD. These data indicate that coronary monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO do not predict the risk of progression of CAD.
Keywords coronary artery disease      inflammation      integrin      myeloperoxidase      leukocyte activation     
Corresponding Author(s): Marijke A. de Vries   
Just Accepted Date: 14 January 2016   Online First Date: 18 February 2016    Issue Date: 31 March 2016
 Cite this article:   
Marijke A. de Vries,Arash Alipour,Erwin Birnie, et al. Coronary leukocyte activation in relation to progression of coronary artery disease[J]. Front. Med., 2016, 10(1): 85-90.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-016-0435-1
https://academic.hep.com.cn/fmd/EN/Y2016/V10/I1/85
Total group (n = 99) Progression CAD (n = 13) No progression CAD (n = 86) P value
Age (year) 65±12 67±11 64±12 0.50
Gender (% male) 60 62 60 >0.99
CAD (%) 71 100 66 0.009
DM (%) 28 15 30 0.34
Smoking (%) 14 15 14 >0.99
Statin use (%) 68 92 64 0.06
ASA use (%) 67 92 63 0.06
ACE-i use (%) 33 23 35 0.54
BMI (kg/m2) 27.6±4.1 25.5±4.6 27.9±4.0 0.06
SBP (mmHg) 144±22 154±27 143±21 0.09
Glucose (mmol/L) 6.8±1.7 6.5±1.3 6.8±1.8 0.59
LDL-c (mmol/L) 2.8±1.0 2.8±1.2 2.8±1.0 0.95
HDL-c (mmol/L) 1.3±0.3 1.3±0.3 1.2±0.3 0.56
Triglycerides (mmol/L) 1.55 (1.07?2.19) 1.46 (0.90??1.78) 1.56 (1.08?2.25) 0.43
CRP (mg/L) 2.0 (1.0?4.0) 1.0 (1.0?5.0) 2.0 (1.0?4.0) 0.64
Leukocyte count (109/L) 7.1 (6.0?8.3) 7.1 (5.6?7.7) 7.1 (6.1?8.4) 0.32
Complement C3 (g/L) 1.23±0.24 1.28±0.24 1.22±0.24 0.39
Mono CD11b LCA (au) 31.8 (25.5?40.8) 28.9 (21.0?42.4) 32.2 (25.6?40.7) 0.66
Mono CD11b RCA (au) 32.2 (26.5?41.8) 29.8 (23.0?42.0) 32.2 (26.7?41.7) 0.56
Neutro CD11b LCA (au) 30.9 (24.3?38.6) 29.0 (22.2?38.8) 31.8 (24.4?38.6) 0.51
Neutro CD11b RCA (au) 31.5 (25.6?37.6) 23.7 (20.0?43.8) 31.8 (26.2?37.6) 0.26
Neutro CD66b LCA (au) 7.2 (6.0?9.0) 7.6 (5.5?9.8) 7.1 (6.1?8.9) 0.70
Neutro CD66b RCA (au) 7.3 (5.8?9.3) 7.7 (5.0?11.3) 7.3 (5.8?9.0) 0.72
MPO LCA (au) 162.5 (130.2?202.9) 160.4 (136.4?169.4) 169.6 (129.8?205.0) 0.69
MPO RCA (au) 165.3 (130.1?194.7) 166.9 (147.1?177.3) 164.0 (129.3?196.5) 0.99
Tab.1  Baseline characteristics of study participants (n = 99)
HR (95% CI) P value
Monocyte CD11b in LCA 0.97 (0.94 – 1.04) 0.61
Monocyte CD11b in RCA 0.97 (0.90 – 1.04) 0.42
Neutrophil CD11b in LCA 1.00 (0.94 – 1.06) 0.87
Neutrophil CD11b in RCA 0.97 (0.90 – 1.04) 0.38
Neutrophil CD66b in LCA 1.10 (0.86 – 1.34) 0.45
Neutrophil CD66b in RCA 1.06 (0.82 – 1.37) 0.65
Myeloperoxidase in LCA 1.00 (0.99 – 1.01) 0.95
Myeloperoxidase in RCA 1.00 (0.99 – 1.01) 0.70
Tab.2  Coronary leukocyte activation and risk of future CAD
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