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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

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Front. Mater. Sci.    2014, Vol. 8 Issue (4) : 354-362    https://doi.org/10.1007/s11706-014-0270-8
RESEARCH ARTICLE
Fabrication and characterization of curcumin-loaded silk fibroin/P(LLA-CL) nanofibrous scaffold
Yuan LIAN1,Jian-Chao ZHAN1,Kui-Hua ZHANG1,*(),Xiu-Mei MO2
1. College of Materials and Textile Engineering, Jiaxing University, Jiaxing 314001, China
2. Biomaterials and Tissue Engineering Laboratory, College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China
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Abstract

Curcumin exhibited excellent properties including antioxidant, anti-inflammatory, antiviral, antibacterial, antifungal, anticancer, and anticoagulant activities. In this study, curcumin was incorporated into silk fibroin (SF)/poly(L-lactic acid-co-e-caprolactone) (P(LLA-CL)) nanofibrous scaffolds via electrospinning, and changes brought about by raising the curcumin content were observed: SEM images showed that the average nanofibrous diameter decreased at the beginning and then increased, and the nanofibers became uniform; FTIR showed that the conformation of SF transforming from random coil form to β-sheet structure had not been induced, while SF conformation converted to β-sheet after being treated with 75% ethanol vapor; XRD results confirmed that the crystal structure of (P(LLA-CL)) had been destroyed; The mechanical test illustrated that nanofibrous scaffolds still maintained good mechanical properties. Further, curcumin-loaded nanofibrous scaffolds were evaluated for drug release, antioxidant and antimicrobial activities in vitro. The results showed that curcumin presented a sustained release behavior from nanofibrous scaffolds and maintained its free radical scavenging ability, and such scaffolds could effectively inhibit S. aureus growth (> 95%). Thus, curcumin-loaded SF/P(LLA-CL) nanofibrous scaffolds might be potential candidates for wound dressing and tissue engineering scaffolds.
Keywords curcumin      SF/P(LLA-CL)      nanofibrous scaffold      control release     
Corresponding Author(s): Kui-Hua ZHANG   
Online First Date: 24 November 2014    Issue Date: 04 December 2014
 Cite this article:   
Yuan LIAN,Jian-Chao ZHAN,Kui-Hua ZHANG, et al. Fabrication and characterization of curcumin-loaded silk fibroin/P(LLA-CL) nanofibrous scaffold[J]. Front. Mater. Sci., 2014, 8(4): 354-362.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-014-0270-8
https://academic.hep.com.cn/foms/EN/Y2014/V8/I4/354
Fig.1  SEM images and diameter distributions of nanofibers with different curcumin content: (a)(a′) 0.0; (b)(b′) 2.0% (w/w); (c)(c′) 4.0% (w/w); (d)(d′) 6.0% (w/w).
Fig.2  FTIR results of (a) curcumin and (b) SF/P(LLA-CL) nanofibrous scaffolds: SF/P(LLA-CL) nanofibers (i); curcumin-loaded SF/P(LLA-CL) nanofibers (ii); curcumin-loaded SF/P(LLA-CL) nanofibers after being treated with 75% (v/v) ethanol vapor (iii).
Fig.3  XRD patterns of (a) curcumin and (b) SF/P(LLA-CL) nanofibrous scaffolds: SF/P(LLA-CL) nanofibers (i); curcumin-loaded SF/P(LLA-CL) nanofibers (ii); curcumin-loaded SF/P(LLA-CL) nanofibers after being treated with 75% (v/v) ethanol vapor (iii).
Fig.4  Mechanical properties of SF/P(LLA-CL) nanofibrous scaffolds with different curcumin contents: (a) 0.0; (b) 2.0% (w/w); (c) 4.0% (w/w); (d) 6.0% (w/w).
Curcumin content /(%, w/w) Specimen thickness /mm Elongation at break /% Tensile strength /MPa
0 0.138±0.021 87.02±5.34 4.64±0.32
2.0 0.118±0.010 79.91±7.78 4.38±0.17
4.0 0.099±0.017 83.86±5.87 5.00±0.13
6.0 0.102±0.012 117.44±1.35 5.27±0.34
Tab.1  Mechanical properties of SF/P(LLA-CL) nanofibrous scaffolds with various curcumin contents
Fig.5  Release curves of curcumin from SF/P(LLA-CL) nanofibrous scaffolds in vitro.
Fig.6  Radical scavenging activity of SF/P(LLA-CL) nanofibrous scaffolds with different curcumin content (n = 3).
Content of curcumin /(%, w/w) Bacterial inhibition /%
0.0 15.8±0.53
2.0 98.3±0.29
4.0 99.3±0.13
6.0 99.7±0.85
Tab.2  Bacterial inhibition percentage of SF/P(LLA-CL) nanofiber scaffolds with different curcumin content (n = 3)
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