<i>In vitro</i> expression and analysis of the 826 human G protein-coupled receptors

doi:10.1007/s13238-016-0263-8

Protein Cell

2016, Vol. 7

Issue (5) : 325-337 https://doi.org/10.1007/s13238-016-0263-8

RESEARCH ARTICLE

In vitro expression and analysis of the 826 human G protein-coupled receptors

Xuechen Lv¹,Junlin Liu¹,Qiaoyun Shi¹,Qiwen Tan¹,Dong Wu¹,John J. Skinner¹,Angela L. Walker²,Lixia Zhao¹,Xiangxiang Gu¹,Na Chen¹,Lu Xue¹,Pei Si¹,Lu Zhang¹,Zeshi Wang¹,Vsevolod Katritch²,Zhi-jie Liu¹,Raymond C. Stevens^1,^*(

)

¹. iHuman Institute, ShanghaiTech University, Shanghai 201210, China
². Department of Biological Sciences, Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA

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Abstract

G protein-coupled receptors (GPCRs) are involved in all humanphysiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826humanGPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility.

Keywords G protein-coupled receptors insect protein expression surface expression analysis fusion construct

Corresponding Author(s): Raymond C. Stevens

Issue Date: 27 May 2016

Cite this article:

Xuechen Lv,Junlin Liu,Qiaoyun Shi, et al. In vitro expression and analysis of the 826 human G protein-coupled receptors[J]. Protein Cell, 2016, 7(5): 325-337.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-016-0263-8
https://academic.hep.com.cn/pac/EN/Y2016/V7/I5/325

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[1]	Kai Yu,Yang Yu,Xiaoyan Tang,Huimin Chen,Junyu Xiao,Xiao-Dong Su. Transcriptome analyses of insect cells to facilitate baculovirus-insect expression[J]. Protein Cell, 2016, 7(5): 373-382.

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