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Protein Cell    2024, Vol. 15 Issue (5) : 331-363    https://doi.org/10.1093/procel/pwad051
Applications of genetic code expansion technology in eukaryotes
Qiao-ru Guo1, Yu J. Cao1,2()
1. State Key Laboratory of Chemical Oncogenomic, Guangdong Provincial Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, China
2. Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China
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Abstract

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to proteins. In eukaryotes, genetic code expansion (GCE) enables the incorporation of UAAs and facilitates posttranscriptional modification (PTM), which is not feasible in prokaryotic systems. GCE is also a powerful tool for cell or animal imaging, the monitoring of protein interactions in target cells, drug development, and switch regulation. Therefore, there is keen interest in utilizing GCE in eukaryotic systems. This review provides an overview of the application of GCE in eukaryotic systems and discusses current challenges that need to be addressed.
Keywords genetic code expansion      unnatural amino acid      eukaryotes      basic research      therapeutic applications     
Corresponding Author(s): Yu J. Cao   
Issue Date: 27 May 2024
 Cite this article:   
Qiao-ru Guo,Yu J. Cao. Applications of genetic code expansion technology in eukaryotes[J]. Protein Cell, 2024, 15(5): 331-363.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1093/procel/pwad051
https://academic.hep.com.cn/pac/EN/Y2024/V15/I5/331
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