Biomimetic mineralization and cytocompatibility of nanorod hydroxyapatite/graphene oxide composites

doi:10.1007/s11705-018-1708-9

Front. Chem. Sci. Eng.

2018, Vol. 12

Issue (4) : 798-805 https://doi.org/10.1007/s11705-018-1708-9

RESEARCH ARTICLE

Biomimetic mineralization and cytocompatibility of nanorod hydroxyapatite/graphene oxide composites

Peizhen Duan¹, Juan Shen^1,²(

), Guohong Zou¹, Xu Xia¹, Bo Jin²(

)

¹. School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
². State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials, Southwest University of Science and Technology, Mianyang 621010, China

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Abstract

Nanorod hydroxyapatite (NRHA)/graphene oxide (GO) composites with weight ratios of 0.4, 1.5, and 5 have been fabricated by a facile ultrasonic-assisted method at room temperature and atmospheric pressure. The chemical structure properties and morphology of the composites were characterized by field emission source scanning electron microscope, X-ray diffraction, transmission electron microscopy, and high-resolution transmission electron microscopy. The results indicate that the NRHA/GO composites have an irregular surface with different degree wrinkles and are stable, and NRHA are well combined with GO. In addition, the biomimetic mineralization mechanism of hydroxyapatite on the NRHA/GO composites in simulated body fluid (SBF) is presented. The presence of a bone-like apatite layer on the composite surface indicate that the NRHA/GO composites facilitate the nucleation and growth of hydroxyapatite crystals in SBF for biomimetic mineralization. Moreover, the NRHA-1.5/GO composite and pure GO were cultured with MC3T3-E1 cells to investigate the proliferation and adhesion of cells. In vitro cytocompatibility evaluation demonstrated that the NRHA/GO composite can act as a good template for the growth and adhesion of cells. Therefore, the NRHA/GO composite could be applied as a GO-based, free-template, non-toxic, and bioactive composite to substitute for a damaged or defect bone.

Keywords hydroxyapatite graphene oxide biomimetic mineralization cytocompatibility

Corresponding Author(s): Juan Shen,Bo Jin

Just Accepted Date: 25 January 2018 Online First Date: 18 April 2018 Issue Date: 03 January 2019

Cite this article:

Peizhen Duan,Juan Shen,Guohong Zou, et al. Biomimetic mineralization and cytocompatibility of nanorod hydroxyapatite/graphene oxide composites[J]. Front. Chem. Sci. Eng., 2018, 12(4): 798-805.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-018-1708-9
https://academic.hep.com.cn/fcse/EN/Y2018/V12/I4/798

Fig.1 FESEM images of the (a) NRHA particles (the boxed portion is the morphology of pure GO), (b) NNRHA-0.4/GO, (c) NNRHA-1.5/GO, and (d) NNRHA-5.0/GO

Fig.2 XRD patterns of the (a) NRHA particles, (b) pure GO, (c) NNRHA-0.4/GO, (d) NNRHA-1.5/GO, and (e) NNRHA-5.0/GO

Fig.3 FESEM micrographs of NNRHA/GO composites after immersion in SBF at 37 ^oC. (a, 4 d; d, 7 d): NNRHA-0.4/GO; (b, 4 d; e, 7 d): NNRHA-1.5/GO; (c, 4 d; f, 7 d): NNRHA-5.0/GO. EDX spectra (Figs. 3(a1?f1)) of mineral deposits on NNRHA/GO composites were obtained from the boxed portions of Figs. 3(a?f), respectively

Fig.4 XRD patterns of NNRHA/GO composites after immersion in SBF at 37 °C. (a, 4 d; d, 7 d): NNRHA-0.4/GO; (b, 4 d; e, 7 d): NNRHA-1.5/GO; (c, 4 d; f, 7 d): NNRHA-5.0/GO

Fig.5 (a) TEM, (b) HRTEM images of NNRHA-1.5/GO composites, and (c,d) TEM images of NNRHA-1.5/GO after immersion in SBF at 37 °C for 7 d

Fig.6 Schematic presentation of the biomimetic mineralization mechanism of HA on NNRHA/GO composites in SBF

Fig.7 MTT assays for the proliferation of MC3T3-E1 cells on pure GO, NNRHA-1.5/GO, and blank control (glass), each of which was cultured for 1, 3, and 5 d under the same culture condition. Error bars represent the standard deviation from the mean±standard deviation, n = 3: *** p<0.001, ** p<0.01

Fig.8 Phase-contrast microscopy photographs of MC3T3-E1 cells (denoted as C) grown on (a, b and c) pure GO, (d, e and f) NNRHA-1.5/GO samples (denoted as S) after culturing for 1, 3 and 5 d

Fig.9 SEM images of the MC3T3-E1 cells after cultured for 3 d on (a and c) pure GO, and (b and d) NNRHA-1.5/GO

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