Enhanced biohydrogen generation from organic
wastewater containing N H 4 + by phototrophic
bacteria Rhodobacter sphaeroides AR-3

doi:10.1007/s11783-009-0154-9

Front.Environ.Sci.Eng.

2009, Vol. 3

Issue (4) : 387-392 https://doi.org/10.1007/s11783-009-0154-9

Research articles

Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3

Guanghong ZHENG,Zhuhui KANG,Yifan QIAN,Lei WANG,

State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;

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Abstract "Graphic"

is typically an inhibitor to hydrogen production from organic wastewater by photo-bacteria. In this experiment, biohydrogen generation with wild-typeanoxygenic phototrophic bacterium Rhodobacter sphaeroideswas found to be sensitive to "Graphic"

due to the significant inhibition of "Graphic"

to its nitrogenase. In order to avoid the inhibition of "Graphic"

to biohydrogen generation by R. sphaeroides, a glutamine auxotrophic mutant R. sphaeroides AR-3 was obtained by mutagenizing with ethyl methane sulfonate. The AR-3 mutant could generate biohydrogen efficiently in the hydrogen production medium with a higher "Graphic"

concentration, because the inhibition of "Graphic"

to nitrogenase of AR-3 was released. Under suitable conditions, AR-3 effectively produced biohydrogen from tofu wastewater with an average generation rate of 14.2 mL/L·h. This generation rate was increased by more than 100% compared with that from wild-type R. sphaeroides.

Keywords ammonium anoxygenic phototrophic bacterium biohydrogen glutamine auxotrophic tofu wastewater

Issue Date: 05 December 2009

Cite this article:

Guanghong ZHENG,Zhuhui KANG,Yifan QIAN, et al. Enhanced biohydrogen generation from organic wastewater containing N H 4 + by phototrophic bacteria Rhodobacter sphaeroides AR-3[J]. Front.Environ.Sci.Eng., 2009, 3(4): 387-392.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-009-0154-9
https://academic.hep.com.cn/fese/EN/Y2009/V3/I4/387

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