Influence of arsanilic acid, Cu2+, PO43− and their interaction on anaerobic digestion of pig manure

doi:10.1007/s11783-017-1004-9

Front. Environ. Sci. Eng.

2018, Vol. 12

Issue (2) : 9 https://doi.org/10.1007/s11783-017-1004-9

RESEARCH ARTICLE

Influence of arsanilic acid, Cu²⁺, PO₄³⁻ and their interaction on anaerobic digestion of pig manure

Ping He¹, Guangxue Wu², Rui Tang¹, Peilun Ji¹, Shoujun Yuan^1,³, Wei Wang^1,³(

), Zhenhu Hu^1,³(

)

¹. School of Civil Engineering, Hefei University of Technology, Hefei 230009, China
². Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
³. Institute of Water Treatment and Wastes Reutilization, Hefei University of Technology, Hefei 230009, China

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Abstract

The methanogenesis was severely inhibited with 0.46 mM ASA addition.

PO₄³⁻ didn’t attenuate the methanogenesis inhibition in the existence of ASA.

ASA was transformed to As(III), As(V), MMA and DMA in anaerobic digestion.

Cu²⁺ mitigated the methanogenesis inhibition via impeding the degradation of ASA.

Arsanilic acid (ASA), copper ion (Cu²⁺) and phosphate (PO₄³⁻) are widely used as feed additives for pigs. Most of these three supplemented feed additives were excreted in feces and urine. Anaerobic digestion is often used for the management of pig manure. However, the interaction of ASA with Cu²⁺ or PO₄³⁻ on anaerobic digestion is still not clear. In this study, the influence of ASA, Cu²⁺, PO₄³⁻ and their interaction on anaerobic digestion of pig manure and the possible mechanisms were investigated. The initial concentrations of ASA, Cu²⁺ and PO₄³⁻ were 0.46 mM, 2 mM and 2 mM in the anaerobic digester, respectively. The methanogenesis was severely inhibited in the assays with only ASA addition, only Cu²⁺ addition and ASA+ PO₄³⁻ addition with the inhibition index of 97.8%, 46.6% and 82.6%, respectively, but the methanogenesis inhibition in the assay with ASA+ Cu²⁺ addition was mitigated with the inhibition index of 39.4%. PO₄³⁻ had no obvious impacts on the degradation of ASA. However, Cu²⁺ addition inhibited the degradation of ASA, mitigating the methanogenesis inhibition. The existence of ASA would inhibit methanogenesis and generate more toxic inorganic arsenic compounds during anaerobic digestion, implying the limitation of anaerobic digestion for ASA- contaminated animal manure. However, the co-existence of ASA and Cu²⁺ could mitigate the inhibition. These results could provide useful information for the management of anaerobic digestion of pig manure containing ASA with Cu²⁺.

Keywords Arsanilic acid (ASA) Methanogenesis Inhibition Copper Phosphate Inorganic arsenics

Corresponding Author(s): Wei Wang,Zhenhu Hu

Issue Date: 26 September 2017

Cite this article:

Ping He,Guangxue Wu,Rui Tang, et al. Influence of arsanilic acid, Cu²⁺, PO₄³⁻ and their interaction on anaerobic digestion of pig manure[J]. Front. Environ. Sci. Eng., 2018, 12(2): 9.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-017-1004-9
https://academic.hep.com.cn/fese/EN/Y2018/V12/I2/9

Tab.1 Experimental design of the anaerobic digestion

Fig.1 Daily methane production (a) and cumulative methane production (b) with pig manure as substrate

Fig.2 Variation of volatile fatty acids concentration during anaerobic digestion of pig manure

Tab.2 Estimated parameters using Gompertz equation for methane production

Fig.3 Biotransformation of ASA during anaerobic digestion of pig manure

Fig.4 Generation of inorganic arsenic (a) and speciation of the degradation products of ASA in aqueous phase at the 8th day, 15th day, 24th day and 60th day (b); ? is for the assay adding ASA, ☆ is for the assay adding ASA and Cu²⁺, ○ is for the assay adding ASA and PO₄³^-

Fig.5 Distribution of total arsenic in supernate and sludge: (a) the assay adding ASA, (b) the assay adding ASA and Cu²⁺ and (c) the assay adding ASA and PO₄³^-

Fig.6 Arsenic species in the sludge after 60-day inoculation

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