Fabrication of carboxylic graphene oxide-composited polypyrrole film for neurite growth under electrical stimulation

doi:10.1007/s11706-019-0467-y

Front. Mater. Sci.

2019, Vol. 13

Issue (3) : 258-269 https://doi.org/10.1007/s11706-019-0467-y

RESEARCH ARTICLE

Fabrication of carboxylic graphene oxide-composited polypyrrole film for neurite growth under electrical stimulation

Chaoyuan LIU, Zhongbing HUANG(

), Ximing PU, Lei SHANG, Guangfu YIN, Xianchun CHEN, Shuang CHENG

College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China

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Abstract

An aligned composite film was fabricated via the deposition of carboxylic graphene oxide (C-GO) and polypyrrole (PPy) nanoparticles on aligned poly(L-lactic acid) (PLLA) fiber-films (named as C-GO/PPy/PLLA), which has the core (PLLA)–sheath (C-GO/PPy) structure, and the composition of C-GO (~4.8 wt.% of PPy sheath) significantly enhanced the tensile strength and the conductivity of the PPy/PLLA film. Especially, after 4 weeks of immersion in the PBS solution, the conductivity and the tensile strength of C-GO/PPy/PLLA films still remained ~6.10 S/cm and 28.9 MPa, respectively, which could meet the need of the sustained electrical stimulation (ES) therapy for nerve repair. Moreover, the neurite length and the neurite alignment were significantly increased through exerting ES on C-GO/PPy/PLLA films due to their sustained conductivity in the fluid of cell culture. These results indicated that C-GO/PPy/PLLA with sustained conductivity and mechanical property possessed great potential of nerve repair by exerting lasting-ES.

Keywords sustained conductivity electrical stimulation carboxylic graphene oxide polypyrrole

Corresponding Author(s): Zhongbing HUANG

Online First Date: 19 July 2019 Issue Date: 29 September 2019

Cite this article:

Chaoyuan LIU,Zhongbing HUANG,Ximing PU, et al. Fabrication of carboxylic graphene oxide-composited polypyrrole film for neurite growth under electrical stimulation[J]. Front. Mater. Sci., 2019, 13(3): 258-269.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-019-0467-y
https://academic.hep.com.cn/foms/EN/Y2019/V13/I3/258

Fig.1 Morphological observation. SEM images of (a) electrospun PLLA fiber-film, (b) PPy/PLLA composite film, (c) C-GO/PPy/PLLA film, and (d) the cross section of C-GO/PPy/PLLA film. The red arrows in (c) and (d) point to C-GO sheets and PPy sheath, respectively, and the blue arrow in (d) points to the inner PLLA fiber.

Fig.2 (a) FTIR results of PLLA, PPy/PLLA and C-GO/PPy/PLLA. (b) XRD patterns of the shells of PPy/PLLA and C-GO/PPy/PLLA.

Fig.3 TG curves of DBS-doped PPy and C-GO/DBS-doped PPy sheaths.

Fig.4 Stability analyses of the films after immersion in PBS for 0, 1, 2 and 4 weeks: typical tensile curves of (a) C-GO/PPy/PLLA and (b) PPy/PLLA film; (c) the tensile strength and (d) the conductivity analyses for two films.

Fig.5 MTT results for (a) L929 cells and (b) PC12 cells cultured on PPy/PLLA and C-GO/PPy/PLLA films.

Fig.6 Cell immunostaining images of PC 12 cells seeded onto the C-GO/PPy/PLLA film (a) with ES and (b) without ES. (c) Average neurite length and (d) cellular alignment angle histograms of corresponding immunostaining images of PC 12 cells.

Fig. S1 Schematic diagram of electrospinning device.

Fig. S2 Schematic of electrochemical deposition.

Fig. S3 Schematic of the device for cells culture under ES through the composite film.

Fig. S4 Contact angle test: contact angle images of (a) PPy/PLLA and (b) C-GO/PPy/PLLA film; (c) the wetting angle comparison of two films.

Fig. S5 SEM images of cross-sections of C-GO/PPy/PLLA film (upper) and PPy/PLLA film (lower) after tensile tests at different immersion time in PBS: (a)(e) 0 week; (b)(f) 1 week; (c)(g) 2 weeks; (d)(h) 4 weeks.

Fig. S6 Cell immunostaining images of PC 12 cells cultured on (a) C-GO/PPy/PLLA film and (b) PPy/PLLA film with ES. (c) Average neurite length and (d) cellular alignment from corresponding immunostaining images of PC 12 cells.

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