Electrospun polypyrrole-coated polycaprolactone nanoyarn nerve guidance conduits for nerve tissue engineering

doi:10.1007/s11706-018-0445-9

Front. Mater. Sci.

2018, Vol. 12

Issue (4) : 438-446 https://doi.org/10.1007/s11706-018-0445-9

RESEARCH ARTICLE

Electrospun polypyrrole-coated polycaprolactone nanoyarn nerve guidance conduits for nerve tissue engineering

Xin PAN^1,², Binbin SUN¹(

), Xiumei MO³(

)

¹. Department of Orthopaedics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
². Sunna Technologies (Shanghai) Co., Ltd., Shanghai 201203, China
³. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China

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Abstract

Nerve guidance conduits (NGCs) can provide suitable microenvironment for nerve repair and promote the proliferation and migration of Schwann cells (SCs). Thus, we developed nerve guidance conduits (NGCs) with polypyrrole-coated polycaprolactone nanoyarns (PPy-PCL-NYs) as fillers in this study. PCL-NYs with the oriented structure were prepared with a double-needle electrospinning system and then PPy was coated on PCL-NYs via the in situ chemical polymerization. Subsequently, PCL nanofibers were collected around nanoyarns by the conventional electrospinning process as the outer layer to obtain PPy-PCL-NY nerve guidance conduits (PPy-PCL-NY NGCs). PPy-PCL-NYs were analyzed by SEM, FTIR and XPS. Results showed that PPy was homogeneously and uniformly deposited on the surface of PCL-NY. Strain–stress curves and the Young’s modulus of PPy-PCL-NYs were investigated compared with those of non-coated PCL-NYs. Studies on biocompatibility with SCs indicated that PPy-PCL-NY NGCs were more conducive to the proliferation of SCs than PCL-NY NGCs. In summary, PPy-PCL-NY NGCs show the promising potential for nerve tissue engineering repair and regeneration.

Keywords electrospinning Schwann cell (SC) polypyrrole (PPy) polycaprolactone nanoyarn (PCL-NY) nerve guidance conduit (NGC)

Corresponding Author(s): Binbin SUN,Xiumei MO

Online First Date: 30 November 2018 Issue Date: 10 December 2018

Cite this article:

Xin PAN,Binbin SUN,Xiumei MO. Electrospun polypyrrole-coated polycaprolactone nanoyarn nerve guidance conduits for nerve tissue engineering[J]. Front. Mater. Sci., 2018, 12(4): 438-446.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-018-0445-9
https://academic.hep.com.cn/foms/EN/Y2018/V12/I4/438

Fig.1 Schematic diagram of PPy-PCL-NY NGCs fabrication process.

Fig.2 Digital and SEM images of (a)(b)(c) PCL-NYs and (d)(e)(f) PPy-PCL-NYs.

Fig.3 SEM images of cross-sections of (a) PCL-NY NGCs, (b) PPy-PCL-NY NGCs, (c) intraductal PCL-NYs, and (d) intraductal PPy-PCL-NYs.

Fig.4 FTIR spectra of PPy, PCL-NYs and PPy-PCL-NYs.

Fig.5 XPS spectra of PCL-NYs and PPy-PCL-NYs.

Tab.1 Surface elemental compositions of PCL-NYs and PPy-PCL-NYs

Fig.6 Stress?strain curves of PCL-NYs and PPy-PCL-NYs.

Tab.2 Mechanical properties of PCL-NYs and PPy-PCL-NYs

Fig.7 MTT results of the SC proliferation on TCP, PCL-NY NGCs and PPy-PCL-NY NGCs after culturing for 1, 3, 5 and 7 d.

Fig.8 (a)(c) HE images and (b)(d) immunofluorescence images with anti-S100 antibody of cross-sections after the SC culture for 7 d on PPy-PCL-NY NGCs (upper) and PCL-NY NGCs (lower).

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