Fabrication and characterization of <i>Antheraea pernyi</i> silk fibroin-blended P(LLA-CL) nanofibrous scaffolds for peripheral nerve tissue engineering

doi:10.1007/s11706-017-0368-x

Front. Mater. Sci.

2017, Vol. 11

Issue (1) : 22-32 https://doi.org/10.1007/s11706-017-0368-x

RESEARCH ARTICLE

Fabrication and characterization of Antheraea pernyi silk fibroin-blended P(LLA-CL) nanofibrous scaffolds for peripheral nerve tissue engineering

Juan WANG¹,Binbin SUN¹,Muhammad Aqeel BHUTTO¹,Tonghe ZHU¹,Kui YU³,Jiayu BAO¹,Yosry MORSI⁴,Hany EL-HAMSHARY^5,⁶,Mohamed EL-NEWEHY^5,⁶,Xiumei MO^1,²(

)

¹. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
². Shandong International Biotechnology Park Development Co., Ltd., Yantai 264670, China
³. College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
⁴. Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Vic 3122, Australia
⁵. Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
⁶. Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt

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Abstract

Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P(LLA-CL) nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P(LLA-CL). Characterization of ¹³C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P(LLA-CL). The tensile strength and elongation at break increased with the content increase of P(LLA-CL). The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApF/P(LLA-CL) blended nanofibrous scaffolds significantly promoted cell growth as compare to P(LLA-CL), especially when the weight ratio of ApF to P(LLA-CL) was 25:75. The present work provides a basis for further studies of this novel nanofibrous material (ApF/P(LLA-CL)) in peripheral nerve tissue repair or regeneration.

Keywords ApF/P(LLA-CL) electrospinning nanofibers scaffolds Schwann cells peripheral nerve tissue engineering

Corresponding Author(s): Xiumei MO

Online First Date: 09 January 2017 Issue Date: 22 January 2017

Cite this article:

Juan WANG,Binbin SUN,Muhammad Aqeel BHUTTO, et al. Fabrication and characterization of Antheraea pernyi silk fibroin-blended P(LLA-CL) nanofibrous scaffolds for peripheral nerve tissue engineering[J]. Front. Mater. Sci., 2017, 11(1): 22-32.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-017-0368-x
https://academic.hep.com.cn/foms/EN/Y2017/V11/I1/22

Fig.1 SEM images and diameter distributions of electrospun ApF/P(LLA-CL) (50:50) blend nanofibers at different concentrations: (a) 6% (w/v); (b) 8% (w/v); (c) 10% (w/v); (d) 12% (w/v).

Fig.2 SEM images and diameter distributions of 10% (w/v) ApF/P(LLA-CL) solution with different blend weight ratios of w(ApF):w(P(LLA-CL)): (a) 100:0; (b) 75:25; (c) 50:50; (d) 25:75; (e) 0:100.

Fig.3 Structural analyses of ApF/P(LLA-CL) blended nanofibers (100:0 (i); 75:25 (ii); 50:50 (iii); 25:75 (iv); 0:100 (v)): (a) ATR-FTIR spectra; (b)¹³C CP/MAS NMR spectra.

Fig.4 Water contact angle measurement on electrospun nanofibers surface of ApF/P(LLA-CL) blended nanofibrous scaffolds with different w(ApF):w(P(LLA-CL)) ratios.

Fig.5 Mechanical properties of ApF/P(LLA-CL) blended nanofibrous scaffolds with different w(ApF):w(P(LLA-CL)) ratios: (a) tensile stress–strain relationship; (b) elongation at break; (c) tensile strength. The data represent the mean±SD (n = 5), and the symbol “*” represents statistically significant differences (p<0.05) compared with pure P(LLA-CL) (w(ApF):w(P(LLA-CL)) = 0:100).

Fig.6 Proliferation of SCs cultured on different nanofibrous scaffolds for 1, 3, 5 and 7 d. Data are expressed as mean±SD (n = 3). The symbol “*” represents statistically significant differences (p<0.05).

Fig.7 SEM images of SCs grown for 3 d on ApF/P(LLA-CL) nanofibrous scaffolds and TCP with different w(ApF):w(P(LLA-CL)) ratios: (a) 100:0 (pure ApF); (b) 75:25; (c) 50:50; (d) 25:75; (e) 0:100 (pure P(LLA-CL)); (f) TCP.

Fig.8 Immunochemistry staining images of SCs after a 3-day culture on different nanofibrous scaffolds. Nucleus was stained by DAPI (blue) and cytoplasm was stained by rhodamine phalloidin (red).

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