<i>In vitro</i> evaluation of electrospun gelatin–glutaraldehyde nanofibers

doi:10.1007/s11706-016-0329-9

Front. Mater. Sci.

2016, Vol. 10

Issue (1) : 90-100 https://doi.org/10.1007/s11706-016-0329-9

RESEARCH ARTICLE

In vitro evaluation of electrospun gelatin–glutaraldehyde nanofibers

Jianchao ZHAN^1,²,Yosry MORSI³,Hany EI-HAMSHARY^4,⁵,Salem S. AL-DEYAB⁴,Xiumei MO^1,^*(

)

1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
2. College of Materials and Textile Engineering, Jiaxing University, Jiaxing 314001, China
3. Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, Hawthorn, Vic 3122, Australia
4. Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
5. Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt

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Abstract

The gelatin–glutaraldehyde (gelatin–GA) nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity. The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolytic resistance, mechanical property and biocompatibility of nanofiber scaffolds were tested and characterized. The gelatin–GA nanofiber has nice uniform diameter and more than 80% porosity. The hydrolytic resistance and mechanical property of the gelatin–GA nanofiber scaffolds are greatly improved compared with that of gelatin nanofibers. The contact angle, moisture absorption, hydrolysis resistance, thermal resistance and mechanical property of gelatin–GA nanofiber scaffolds could be adjustable by varying the gelatin solution concentration and GA content. The gelatin–GA nanofibers had excellent properties, which are expected to be an ideal scaffold for biomedical and tissue engineering applications.

Keywords nanofiber electrospinning gelatin tissue engineering

Corresponding Author(s): Xiumei MO

Online First Date: 08 January 2016 Issue Date: 15 January 2016

Cite this article:

Jianchao ZHAN,Yosry MORSI,Hany EI-HAMSHARY, et al. In vitro evaluation of electrospun gelatin–glutaraldehyde nanofibers[J]. Front. Mater. Sci., 2016, 10(1): 90-100.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-016-0329-9
https://academic.hep.com.cn/foms/EN/Y2016/V10/I1/90

Fig.1 Preparation process of gelatin–GA nanofiber scaffolds.

Fig.2 SEM images of gelatin–GA nanofiber scaffolds and corresponding diameter distribution diagrams: (a) G64; (b) G63; (c) G74; (d) G73; (e) G84; (f) G83.

Tab.1 The physical properties of gelatin–GA nanofiber scaffolds

Fig.3 FTIR spectra of gelatin–GA nanofibers with the mass ratio of w(GA)/w(gelatin) at (a) 1/400 and (b) 1/300.

Fig.4 XRD patterns of gelatin–GA nanofiber scaffolds.

Fig.5 DSC curves of gelatin–GA nanofiber scaffold. All the samples possess a similar water content of 12 wt.%.

Fig.6 Water-holding capacity–soak time curves of gelatin–GA nanofiber scaffolds.

Fig.7 Water evaporation rate–time curves of gelatin–GA nanofiber scaffolds.

Fig.8 Contact angles of the gelatin–GA electrospun nanofiber scaffolds.

Fig.9 Degradation curves of the gelatin–GA nanofiber scaffolds.

Fig.10 Stress–strain curves of the gelatin–GA nanofiber scaffolds.

Fig.11 Proliferation of L929 on TCP and gelatin–GA nanofiber scaffolds after 1, 3, 5 and 7 d.

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