In vivo and in vitro development of Tibetan antelope (Pantholops hodgsonii) interspecific cloned embryos

doi:10.15302/J-FASE-2014012

Front. Agr. Sci. Eng.

2014, Vol. 1

Issue (1) : 28-36 https://doi.org/10.15302/J-FASE-2014012

RESEARCH ARTICLE

In vivo and in vitro development of Tibetan antelope (Pantholops hodgsonii) interspecific cloned embryos

Guanghua SU,Lei CHENG,Yu GAO,Kun LIU,Zhuying WEI,Chunling BAI,Fengxia YIN,Li GAO,Guangpeng LI,Shorgan BOU(

)

The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of Agriculture, Inner Mongolia University, Hohhot 010021, China

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Abstract

The Tibetan antelope is endemic to the Tibetan Plateau, China, and is now considered an endangered species. As a possible rescue strategy, the development of embryos constructed by interspecies somatic cell nuclear transfer (iSCNT) was examined. Tibetan antelope fibroblast cells were transferred into enucleated bovine, ovine and caprine oocytes. These cloned embryos were then cultured in vitro or in the oviducts of intermediate animals.

Less than 0.5% of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst stage. However, when the cloned antelope-bovine embryos were transferred to caprine oviducts, about 1.6% of the embryos developed to the blastocyst stage. In contrast, only 0.7% of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine oviducts. The treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and caprine. When the antelope-bovine embryos, constructed from oocytes treated with roscovitine or trichostatin A, were cultured in rabbit oviducts 2.3% and 14.3% developed to blastocysts, respectively. It is concluded that although some success was achieved with the protocols used, interspecies cloning of Tibetan antelope presents difficulties still to be overcome. The mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

Keywords interspecific nuclear transfer bovine ovine caprine oviduct apoptosis

Corresponding Author(s): Shorgan BOU

Issue Date: 22 May 2014

Cite this article:

Guanghua SU,Lei CHENG,Yu GAO, et al. In vivo and in vitro development of Tibetan antelope (Pantholops hodgsonii) interspecific cloned embryos[J]. Front. Agr. Sci. Eng. , 2014, 1(1): 28-36.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2014012
https://academic.hep.com.cn/fase/EN/Y2014/V1/I1/28

Fig.1 Karyotype analyses of Tibetan antelope, bovine, caprine and ovine. (a) Tibetan antelope, 2n = 60; (b) bovine, 2n = 60; (c) caprine, 2n = 60; and (d) ovine, 2n = 54. ( × 1000).

Fig.2 Representative images of the Tibetan antelope interspecific SCNT embryos cultured in vitro or in vivo. (a) Antelope-bovine blastocyst derived from roscovitine-treated oocytes and incubated in rabbit oviduct; (b) antelope-bovine blastocyst derived from TSA-treated oocytes and incubated in rabbit oviduct; (c) antelope-bovine blastocyst incubated in caprine oviduct; (d) blastocyst obtained from in vitro culture; (e) 8- to 16-cell stage antelope-ovine cloned embryos cultured in vitro; (F) 8- to 16-cell stage antelope-bovine cloned embryos cultured in vitro. Each scale bar represents 100 μm.

Tab.1 The in vitro development of the Tibetan antelope interspecific cloned embryos

Tab.2 The in vivo development in intermediate animal oviducts of the Tibetan antelope interspecific cloned embryos

Tab.3 Oviduct in vivo development of Tibetan antelope interspecific embryos from ROS- and TSA-treated oocytes

Fig.3 Apoptosis in interspecific cloned embryos. (a) Antelope-bovine morulae cultured in vitro; (b) antelope-bovine morulae cultured in vivo. TUNEL stain or apoptosis appears green and nucleic acid appears red. The arrows indicate the sites of apoptosis. Each scale bar represents 100 μm.

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