Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications

doi:10.1007/s11705-012-1209-1

Front Chem Sci Eng

2012, Vol. 6

Issue (3) : 339-347 https://doi.org/10.1007/s11705-012-1209-1

RESEARCH ARTICLE

Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications

Dongjiao ZHOU¹, Shaochuan SHEN¹, Junxian YUN¹(

), Kejian YAO¹, Dong-Qiang LIN²(

)

1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China; 2. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

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Abstract

Dextran-hyaluronate (Dex-HA) based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate (Dex-MA) and hyaluronate methacrylate (HA-MA) at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concentration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% (w/w) at the HA-MA concentration of 1% (w/v), which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% (w/w) macromonomer solution had high water permeability (5.1 × 10^-12 m²) and high porosity (92.4%). The pore diameter examined by scanning electron microscopy (SEM) was in a broad range of 50–135 μm with the mean pore diameter of 91 μm. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.

Keywords cryogel scaffold tissue engineering dextran hyaluronate 3T3-L1 preadipocyte

Corresponding Author(s): YUN Junxian,Email:yunjx@zjut.edu.cn; LIN Dong-Qiang,Email:lindq@zju.edu.cn

Issue Date: 05 September 2012

Cite this article:

Dongjiao ZHOU,Shaochuan SHEN,Junxian YUN, et al. Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications[J]. Front Chem Sci Eng, 2012, 6(3): 339-347.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1209-1
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I3/339

Fig.1 H-NMR spectra of Dex-MA (a) and HA-MA (b) dissolved in DO

Fig.2 Experimental and fitted flow rates as a function of pressure drop for 4%, 5% and 6% cryogel scaffold samples

Fig.3 The equilibrium mass swelling ratios of dry cryogel scaffolds (4%, 5% and 6%) in deionized water at 37°C

Fig.4 Microstructure of 4% (a), 5% (b) and 6% (c) cryogel scaffolds at 150 × magnification

Tab.1 Pore size distributions, mean pore diameters and porosities of cryogel scaffolds (4% - 6%)

Fig.5 Compressive stress-strain behavior of 4%, 5% and 6% hydrated cryogel scaffold samples

Fig.6 Morphology of 3T3-L1 preadipocytes within the scaffold. Cell growth observed by SEM (a, c) 1000 × magnification and inverted microscope (b, d) at 100 × magnification 6 days (a, b) and 17 days (c, d) post-seeding incubation

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