Biosorption of mercury and lead by aqueous <italic>Streptomyces</italic> VITSVK9 sp. isolated from marine sediments from the bay of Bengal, India

doi:10.1007/s11705-012-1285-2

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 198-202 https://doi.org/10.1007/s11705-012-1285-2

RESEARCH ARTICLE

Biosorption of mercury and lead by aqueous Streptomyces VITSVK9 sp. isolated from marine sediments from the bay of Bengal, India

Pratibha SANJENBAM, Kumar SAURAV, Krishnan KANNABIRAN(

)

Biomolecules and Genetics Division, School of Biosciences and Technology, VIT University, Vellore 632014, India

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Abstract

Toxic heavy metals are increasingly accumulating in the environment worldwide and are considered to be life threatening contaminants. The biosorption of mercury and lead by marine actinomycetes isolated from marine sediment collected from the Bay of Bengal coast of Puducherry, India, was evaluated. The maximum tolerance concentration (MTC) of Streptomyces sp. was determined by a well diffusion method and a broth dilution method. The effects of the initial metal ion concentration, the pH and the biomass dosage on the biosorption of mercury and lead ions were investigated. The MTC of the isolate to metals was 200 mg·L^-1 for mercury and 1800 mg·L^-1 for lead. At neutral pH, the isolate had a maximum biosorption of metal ions of 200 mg·L^-1 and 150 mg·L^-1 for mercury and lead respectively. Fourier transform infrared (FTIR) absorption spectra showed the chemical interactions between the functional groups in the biomass such as hydroxyl (-OH), amine (-NH₂), carboxyl (-COOH) and the metal ions. The isolate was further characterized by molecular taxonomy and identified as a member of the genus Streptomyces. Based on the phenotypic and phylogenetic analysis, the strain was classified as a new species of the genus Streptomyces and designated as Streptomyces VITSVK9 sp. (HM137310). A blast search of the 16S rDNA sequence of the strain showed the most similarity (95%) with Streptomyces sp. A515 Ydz-FQ (EU384279). Based on the results, it can be concluded that this marine Streptomyces could be used as a biosorbent for the removal of heavy metal ions from aqueous environments.

Keywords mercury lead biosorption maximum tolerance concentration Streptomyces VITSVK9 sp.

Corresponding Author(s): KANNABIRAN Krishnan,Email:kkb@vit.ac.in

Issue Date: 05 June 2012

Cite this article:

Pratibha SANJENBAM,Kumar SAURAV,Krishnan KANNABIRAN. Biosorption of mercury and lead by aqueous Streptomyces VITSVK9 sp. isolated from marine sediments from the bay of Bengal, India[J]. Front Chem Sci Eng, 2012, 6(2): 198-202.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1285-2
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/198

Tab.1 Morphological and biochemical characteristics of the isolate VITSVK9 sp.

Fig.1 Scanning electron micrograph of VITSVK9 sp. showing the smooth surface of spiral spore morphology

Fig.2 Maximum-parsimony tree based on 16S rDNA sequences showing the relationship of VITSVK9 sp. with other species. Only boot strap values of 50 and above are indicated.

Fig.3 Effect of biomass dosage on the biosorption of Hg (II) and Pb (II) by VITSVK9 sp. biomass

Fig.4 Effect of metal ion concentration on the biosorption of Hg (II) and Pb (II) by VITSVK9 sp. biomass

Fig.5 Effect of pH on the biosorption of Hg (II) and Pb (II) by VITSVK9 sp. biomass

Fig.6 FTIR spectra of sp.VITSVK9.
(a) lyophilised native culture biomass; (b) mercury loaded biomass; (c) lead loaded biomass

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