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Frontiers of Earth Science

ISSN 2095-0195

ISSN 2095-0209(Online)

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Front. Earth Sci.    2020, Vol. 14 Issue (4) : 783-788    https://doi.org/10.1007/s11707-020-0829-x
RESEARCH ARTICLE
Different patterns of molecular, carbon and hydrogen isotope compositions of n-alkanes between heterotrophic plant and its hosts
Xin YANG1, Xianyu HUANG1,2()
1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430078, China
2. Hubei Key Laboratory of Critical Zone Evolution, School of Geography and Information Engineering, China University of Geosciences, Wuhan 430078, China
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Abstract

This study investigates the molecular, carbon, and hydrogen isotope compositions of n-alkanes in eight pairs of a holoparasitic plant (Cuscuta chinensis) and its hosts. It is unexpected that C. chinensis has a higher concentration of n-alkanes than its hosts in seven of the eight pairs, and it is preferentially dominated by n-C29 alkane (representing>75% of the total n-alkanes). In addition, the d13C values of C29 alkane in C. chinensis are less negative (avg. 1.8‰) than those in the hosts, while the d2H values of C29 alkane are more negative in C. chinensis (the difference averages 48‰). We propose that the 2H-depletion of n-alkanes in the stem parasite C. chinensis may result from the utilization of stem water with less influence from evapotranspiration or the use of newly synthesized carbohydrates which is 2H-depleted relative to stored sugars. These results highlight the importance of plant nutrient status on the molecular and isotopic compositions of leaf waxes, which shed light on the (paleo)ecological potential of leaf wax d2H values.
Keywords heterotrophic plant      Cuscuta      n-alkane      carbon isotope      hydrogen isotope     
Corresponding Author(s): Xianyu HUANG   
Online First Date: 09 December 2020    Issue Date: 08 January 2021
 Cite this article:   
Xin YANG,Xianyu HUANG. Different patterns of molecular, carbon and hydrogen isotope compositions of n-alkanes between heterotrophic plant and its hosts[J]. Front. Earth Sci., 2020, 14(4): 783-788.
 URL:  
https://academic.hep.com.cn/fesci/EN/10.1007/s11707-020-0829-x
https://academic.hep.com.cn/fesci/EN/Y2020/V14/I4/783
Fig.1  Distribution of n-alkanes in Cuscuta and its hosts. The yellow twill-filled bars indicate Cuscuta, and the green bars indicate their hosts. (a) Sapium sebiferum; (b) Alangium chinense; (c) Debregeasia orientalis; (d) Boehmeria nivea; (e) Alchornea davidii; (f) Parthenocissus dalzielii; (g) Vitis amurensis; (h) Ampelopsis delavayana.
Fig.2  d13C29 values (a) and d2H29 values (b) in Cuscuta (pink bars) and its hosts (black bars: tree; dark bars: shrub; gray bars: vine), together with the differences of d13C29 and d2H29 values between Cuscuta and its hosts.
Sample pair Species C23 C24 C25 C26 C27 C28 C29 C30 C31 C32 C33 Sum ACLa CPIb
Pair-1 Cuscuta 0.0 12.6 1.6 19.6 1.2 37.4 670.9c 16.5 43.6 0.5 1.3 805.2 28.9 8.3
Sapium sebiferum 1.5 1.5 6.1 2.9 14.2 5.0 71.9 4.7 27.0 1.2 3.3 139.4 28.9 8.0
Pair-2 Cuscuta 7.1 2.3 14.9 4.1 32.6 13.8 273.9 4.2 13.0 0.0 0.0 365.8 28.5 14.0
Alangium chinense 0.6 0.5 2.1 1.1 9.5 4.2 57.5 4.7 29.2 1.4 2.9 113.8 29.3 8.4
Pair-3 Cuscuta 13.8 3.5 34.7 10.0 124.2 45.6 1171.6 18.6 99.9 0.0 0.0 1521.9 28.8 18.6
Debregeasia orientalis 2.0 0.8 5.7 0.9 21.8 8.6 475.2 46.8 1804.1 70.9 491.5 2930.9 31.0 20.0
Pair-4 Cuscuta 0.0 1.0 0.3 5.5 2.2 26.5 370.0 10.7 25.9 0.4 0.4 442.9 29.0 9.0
Boehmeria nivea 0.7 0.4 3.7 2.4 29.6 10.5 469.3 27.0 831.5 20.3 182.3 1577.6 30.5 23.6
Pair-5 Cuscuta 0.0 2.7 1.4 8.7 4.7 34.8 472.4 8.4 26.7 0.3 0.4 560.4 29.0 9.2
Parthenocissus dalzielii 3.2 3.5 6.6 7.7 10.9 10.3 40.0 36.4 17.6 1.6 1.6 139.8 28.7 1.3
Pair-6 Cuscuta 0.0 1.5 0.5 7.4 2.4 46.5 635.6 9.4 61.5 0.3 0.5 765.6 29.1 10.8
Vitis amurensis 0.0 0.3 0.4 3.4 3.0 96.8 495.5 27.8 520.3 5.8 18.0 1171.3 29.9 7.7
Pair-7 Cuscuta 0.0 2.3 1.3 8.9 4.0 33.9 508.8 10.2 43.5 0.5 0.8 615.0 29.0 10.0
Ampelopsis delavayana 1.8 2.2 4.9 5.7 13.6 9.9 47.0 6.9 22.9 3.1 5.4 123.7 28.9 3.3
Pair-8 Cuscuta 0.0 1.2 0.8 8.1 2.5 33.3 432.7 9.5 31.7 0.3 0.5 521.3 29.0 8.9
Alchornea davidii 1.1 3.0 3.4 17.4 2.8 17.9 93.7 15.5 55.5 5.8 13.7 233.8 29.4 2.7
Tab.1  Concentration (μg/g) of n-alkanes inthe host-Cuscuta pairs
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