Detecting <italic>Cryptosporidium parvum</italic> and <italic>Giardia lamblia</italic> by coagulation concentration and real-time PCR quantification

doi:10.1007/s11783-012-0455-2

Front Envir Sci Eng

2013, Vol. 7

Issue (1) : 49-54 https://doi.org/10.1007/s11783-012-0455-2

RESEARCH ARTICLE

Detecting Cryptosporidium parvum and Giardia lamblia by coagulation concentration and real-time PCR quantification

Huining ZHANG, Xiaohu Zhang, Shuting ZHANG, Bo WEI, Qipei JIANG(

), Xin YU(

)

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

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Abstract

Rapid and sensitive pathogen detection methods are essential for the effective functioning of the water treatment industry, yet for many pathogens, effective detection and removal methods are still lacking. Cryptosporidium parvum oocysts and Giardia lamblia cysts are two of the most common waterborne pathogens currently infecting the water supply. In this study, a new method was developed for the detection of Cryptosporidium parvum oocysts and Giardia lamblia cysts. The method includes multi-steps as coagulation concentration of (oo)cysts in water, the dissolution of the resulting flocs into a small volume using acid, filtration of the (oo)cysts solution, and DNA extraction, purification, and examination using real-time PCR. The method was tested using spiked tap water and reservoir water as references, and the mean recovery ranged from 19.6% to 97.6% for oocysts, and from 51.4% to 98.7% for cysts. The method is economical and convenient, and is especially suitable for relatively high turbidity surface water.

Keywords Cryptosporidium parvum detection method Giardia lamblia surface water

Corresponding Author(s): JIANG Qipei,Email:cellulose420@163.com; YU Xin,Email:xyu@iue.ac.cn

Issue Date: 01 February 2013

Cite this article:

Huining ZHANG,Shuting ZHANG,Bo WEI, et al. Detecting Cryptosporidium parvum and Giardia lamblia by coagulation concentration and real-time PCR quantification[J]. Front Envir Sci Eng, 2013, 7(1): 49-54.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0455-2
https://academic.hep.com.cn/fese/EN/Y2013/V7/I1/49

Fig.1 Flow chart of the method

Fig.2 oocysts (a) and cysts (b) after 30 min incubation at pH 1

Fig.3 Recovery efficiency of (oo)cysts by coagulation

Fig.4 Recovery efficiencies of (oo)cysts by real-time PCR: (a) ; (b)

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