Enhanced production of laccase by <italic>Coriolus hirsutus</italic> using molasses distillery wastewater

doi:10.1007/s11783-012-0457-0

Front Envir Sci Eng

2013, Vol. 7

Issue (2) : 200-210 https://doi.org/10.1007/s11783-012-0457-0

RESEARCH ARTICLE

Enhanced production of laccase by Coriolus hirsutus using molasses distillery wastewater

Wei SUN^1,2,3,4, Meiying XU^1,2,3, Chunyu XIA^1,2,3, Anhua LI^1,2,3, Guoping SUN^1,2,3(

)

1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China; 2. Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China; 3. State Key Laboratory of Applied Microbiology (Ministry—Guangdong Province Jointly Breeding Base), South China, Guangzhou 510070, China; 4. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China

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Abstract

The effect of physical parameters of cultivation (load volume, temperature, pH, agitation, inoculum size, and incubation period) in the production of laccase by wood-rotting basidiomycete Coriolus hirsutus were studied using diluted molasses distillery wastewater (MDW) as a major composition. Using fractional factorial design, our study first identified load volume, agitation, and inoculum size as statistically significant factors. Optimal preferences and mutual interactions of the factors were then determined by the response surface method, which is based on the center composite design. A quadratic model was used to fit the experimental data. The optimized operational parameters for laccase production were determined to be the following: culture temperature of 25°C, pH 4, load volume of 40 mL diluted MDW in 150 mL flask, agitation rate of 183 r·min^-1, inoculation of 11.5% v/v, and cultivation time of 6 d. The experimental validation under these conditions (the maximum laccase production of 2198.2 U·mL^-1 was within the confidence interval) subsequently verified the accuracy of the constructed model. Moreover, the removal of chemical oxygen demand and nitrogen of MDW reached 62.85% and 48.00% respectively, and the decolorization ratio under the optimal condition was 41.85%. The enhanced production of laccase by C. hirsutus is a new recovery strategy for MDW.

Keywords laccase Coriolus hirsutus fractional factorial design response surface method molasses distillery wastewater

Corresponding Author(s): SUN Guoping,Email:guopingsun@163.com

Issue Date: 01 April 2013

Cite this article:

Wei SUN,Guoping SUN,Meiying XU, et al. Enhanced production of laccase by Coriolus hirsutus using molasses distillery wastewater[J]. Front Envir Sci Eng, 2013, 7(2): 200-210.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0457-0
https://academic.hep.com.cn/fese/EN/Y2013/V7/I2/200

Tab.1 Composition of molasses distillery wastewater

Tab.2 Coded and real values of experiment factors in FFD

Tab.3 Experimental design and response values of FFD ( = 16)

Tab.4 Levels of variables and analysis of the main effect for FFD

Tab.5 Coded and real values of the selected experiment factors

Tab.6 Design and results of the central composite experiment

Tab.7 Variance analysis of the result of the central composite experiment

Fig.1 2D contour plot and corresponding 3D response surface curve for effects of load volume (), agitation (), and their mutual interaction (a); effects of load volume (), inoculum size (), and their mutual interaction (b); effects of agitation (), inoculum size (), and their mutual interaction (c) on laccase production

Fig.2 Metabolism curve of cultivated in MDW

Fig.3 Effect of on quality of MDW

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