Sonication and grinding pre-treatments on <i>Gelidium amansii</i> seaweed for the extraction and characterization of Agarose

doi:10.1007/s11783-018-1040-0

Front. Environ. Sci. Eng.

2018, Vol. 12

Issue (4) : 2 https://doi.org/10.1007/s11783-018-1040-0

RESEARCH ARTICLE

Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose

Kit Wayne Chew^1,², Pau Loke Show^1,²(

), Yee Jiun Yap^2,³, Joon Ching Juan⁴, Siew Moi Phang^5,⁶, Tau Chuan Ling⁶, Jo-Shu Chang⁷

¹. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
². Bioseparation Research Group, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
³. Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
⁴. Nanotechnology & Catalysis Research Centre (NANOCAT), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia
⁵. Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
⁶. Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
⁷. Department of Chemical Engineering, Cheng Kung University, Tainan 701, Taiwan, China

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Abstract

Effects of sonication and grinding pretreatment on agarose quality were observed.

Successful agarose extraction with direct PEG method without the need to dry agar.

FTIR spectra and the characteristics peaks in agarose are explained.

Improvements in gel strength and sulfate content properties in agarose samples.

Various pretreatments methods including sonication and grinding were performed on red seaweed Gelidium amansii for the subsequent extraction of agarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol (PEG) method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique (FTIR), melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarose but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 g cm⁻² and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarose from seaweed and can be applied for future extraction of the agarose.

Keywords Agarose Gelidium Pretreatment Seaweed Sonication

Corresponding Author(s): Pau Loke Show

Issue Date: 26 June 2018

Cite this article:

Kit Wayne Chew,Pau Loke Show,Yee Jiun Yap, et al. Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose[J]. Front. Environ. Sci. Eng., 2018, 12(4): 2.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1040-0
https://academic.hep.com.cn/fese/EN/Y2018/V12/I4/2

Fig.1 FTIR spectra of agarose products from different pretreatment methods

Fig.2 Agarose yield obtained from different pretreatment methods

Tab.1 Gelling and melting temperatures of agarose products from different pretreatment methods

Fig.3 Gel strength of agarose obtained from different pretreatment methods

Fig.4 Sulphate content of agarose obtained from different pretreatment methods

1	Z Al-Nahdi, A Al-Alawi, I Al-Marhobi, A Al-Zefiti (2015). Optimization of yield and chemical properties of agar extracted from Melanothamnus somalensis from Oman sea. Journal of Environmental Science & Engineering, 4: 302–314
2	P Alfred (1967). Fractionation of mixtures of agarose and agaropectin. Ed: Google Patents
3	C Araki (1966). Some recent studies on the polysaccharides of agarophytes. In: Proceedings of the International Seaweed Symposium, 3–17
4	D L Arvizu-Higuera, Y E Rodríguez-Montesinos, J I Murillo-Álvarez, M Muñoz-Ochoa, G Hernández-Carmona (2008). Effect of alkali treatment time and extraction time on agar from Gracilaria vermiculophylla. Journal of Applied Phycology, 20(5): 515–519 https://doi.org/10.1007/s10811-007-9258-4
5	N Azizi, G Najafpour, H Younesi (2017). Acid pretreatment and enzymatic saccharification of brown seaweed for polyhydroxybutyrate (PHB) production using Cupriavidus necator. International Journal of Biological Macromolecules, 101: 1029–1040 https://doi.org/10.1016/j.ijbiomac.2017.03.184 pmid: 28385521
6	F Chemat, N Rombaut, A G Sicaire, A Meullemiestre, A S Fabiano-Tixier, M Abert-Vian (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications: A review. Ultrasonics Sonochemistry, 34: 540–560 https://doi.org/10.1016/j.ultsonch.2016.06.035 pmid: 27773280
7	K W Chew, J C Juan, S M Phang, T C Ling, P L Show (2018). An overview on the development of conventional and alternative extractive methods for the purification of agarose from seaweed. Separation Science and Technology, 53(3): 467–480 https://doi.org/10.1080/01496395.2017.1394881
8	A Chirapart, J Praiboon (2018). Comparison of the photosynthetic efficiency, agar yield, and properties of Gracilaria salicornia (Gracilariales, Rhodophyta) with and without adelphoparasite. Journal of Applied Phycology, 30(1): 149–157
9	K M Cole, R G Sheath (1990). Biology of the Red Algae. Oxford: Cambridge University Press
10	L A Davies, A Dargue, J R Dean, M E Deary (2015). Use of 24 kHz ultrasound to improve sulfate precipitation from wastewater. Ultrasonics Sonochemistry, 23: 424–431 https://doi.org/10.1016/j.ultsonch.2014.08.017 pmid: 25218769
11	J Dodds, F Espitalier, O Louisnard, R Grossier, R David, M Hassoun, F Baillon, C Gatumel, N Lyczko (2007). The Effect of ultrasound on crystallisation–precipitation processes: Some examples and a new segregation model. Particle & Particle Systems Characterization, 24(1): 18–28 https://doi.org/10.1002/ppsc.200601046
12	K S Dodgson (1961). Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochemical Journal, 78(2): 312–319 https://doi.org/10.1042/bj0780312 pmid: 13723349
13	M Duckworth, W Yaphe (1971). Preparation of agarose by fractionation from the spectrum of polysaccharides in agar. Analytical Biochemistry, 44(2): 636–641 https://doi.org/10.1016/0003-2697(71)90253-3 pmid: 5130950
14	G P Fidelis, R B G Camara, M F Queiroz, M S Santos Pereira Costa, P C Santos, H A O Rocha, L S Costa (2014). Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules (Basel, Switzerland), 19(11): 18511–18526 https://doi.org/10.3390/molecules191118511 pmid: 25401396
15	Y Freile-Pelegrín, E Murano (2005). Agars from three species of Gracilaria (Rhodophyta) from Yucatán Peninsula. Bioresource Technology, 96(3): 295–302 https://doi.org/10.1016/j.biortech.2004.04.010 pmid: 15474929
16	P Guerrero, A Etxabide, I Leceta, M Peñalba, K de la Caba (2014). Extraction of agar from Gelidium sesquipedale (Rhodopyta) and surface characterization of agar based films. Carbohydrate Polymers, 99: 491–498 https://doi.org/10.1016/j.carbpol.2013.08.049 pmid: 24274535
17	S Hjertén (1962). A new method for preparation of agaraose for gel electrophoresis. Biochimica et Biophysica Acta, 62(3): 445–449 https://doi.org/10.1016/0006-3002(62)90224-X pmid: 14036248
18	Y Hu, S Wang, Q Wang, Z He, X Lin, S Xu, H Ji, Y Li (2016). Effect of different pretreatments on the thermal degradation of seaweed biomass. Proceedings of the Combustion Institute, 36(2): 2271–2281 https://doi.org/10.1016/j.proci.2016.08.086
19	M Á Hurtado, M Manzano-Sarabia, E Hernández-Garibay, I Pacheco-Ruíz, J A Zertuche-González (2011). Latitudinal variations of the yield and quality of agar from Gelidium robustum (Gelidiales, Rhodophyta) from the main commercial harvest beds along the western coast of the Baja California Peninsula, Mexico. Journal of Applied Phycology, 23(4): 727–734 https://doi.org/10.1007/s10811-010-9572-0
20	Y J Jeon, Y Athukorala, J H Lee (2005). Characterization of agarose product from agar using DMSO. Algae- Korean Phycological Society, 20(1): 61–67 https://doi.org/10.4490/ALGAE.2005.20.1.061
21	E Kolanthai, K Ganesan, M Epple, S N Kalkura (2016). Synthesis of nanosized hydroxyapatite/agarose powders for bone filler and drug delivery application. Materials Today Communications, 8: 31–40 https://doi.org/10.1016/j.mtcomm.2016.03.008
22	P Y Lee, J Costumbrado, C Y Hsu, Y H Kim (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of Visualized Experiments, 62(62): 3923 pmid: 22546956
23	T Matsuhashi (1977). Acid pretreatment of agarophytes provides improvement in agar extraction. Journal of Food Science, 42(5): 1396–1400 https://doi.org/10.1111/j.1365-2621.1977.tb14507.x
24	R Meena, K Prasad, A Siddhanta (2011). Preparation of superior quality products from two Indian agarophytes. Journal of Applied Phycology, 23(2): 183–189 https://doi.org/10.1007/s10811-010-9523-9
25	R Meena, A Siddhanta, K Prasad, B Ramavat, K Eswaran, S Thiruppathi, M Ganesan, V A Mantri, P S Rao (2007). Preparation, characterization and benchmarking of agarose from Gracilaria dura of Indian waters. Carbohydrate Polymers, 69(1): 179–188 https://doi.org/10.1016/j.carbpol.2006.09.020
26	R Mittal, H A Tavanandi, V A Mantri, K S M S Raghavarao (2017). Ultrasound assisted methods for enhanced extraction of phycobiliproteins from marine macro-algae, Gelidium pusillum (Rhodophyta). Ultrasonics Sonochemistry, 38: 92–103 https://doi.org/10.1016/j.ultsonch.2017.02.030 pmid: 28633862
27	M Montingelli, K Benyounis, B Quilty, J Stokes, A Olabi (2017). Influence of mechanical pretreatment and organic concentration of Irish brown seaweed for methane production. Energy, 118: 1079–1089 https://doi.org/10.1016/j.energy.2016.10.132
28	F Pereira-Pacheco, D Robledo, L Rodríguez-Carvajal, Y Freile-Pelegrín (2007). Optimization of native agar extraction from Hydropuntia cornea from Yucatán, México. Bioresource Technology, 98(6): 1278–1284 https://doi.org/10.1016/j.biortech.2006.05.016 pmid: 16797974
29	W Y Seow, C A Hauser (2016). Freeze–dried agarose gels: A cheap, simple and recyclable adsorbent for the purification of methylene blue from industrial wastewater. Journal of Environmental Chemical Engineering, 4(2): 1714–1721 https://doi.org/10.1016/j.jece.2016.02.013
30	M Sharma, J P Chaudhary, D Mondal, R Meena, K Prasad (2015). A green and sustainable approach to utilize bio-ionic liquids for the selective precipitation of high purity agarose from an agarophyte extract. Green Chemistry, 17(5): 2867–2873 https://doi.org/10.1039/C4GC02498B
31	A M Sousa, V D Alves, S Morais, C Delerue-Matos, M P Gonçalves (2010). Agar extraction from integrated multitrophic aquacultured Gracilaria vermiculophylla: Evaluation of a microwave-assisted process using response surface methodology. Bioresource Technology, 101(9): 3258–3267 https://doi.org/10.1016/j.biortech.2009.12.061 pmid: 20056408
32	T J Trivedi, A Kumar (2014). Efficient extraction of agarose from red algae using ionic liquids. Green and Sustainable Chemistry, 4(4): 190–201 https://doi.org/10.4236/gsc.2014.44025
33	L Wang, Z Shen, H Mu, Y Lin, J Zhang, X Jiang (2017). Impact of alkali pretreatment on yield, physico-chemical and gelling properties of high quality agar from Gracilaria tenuistipitata. Food Hydrocolloids, 70: 356–362 https://doi.org/10.1016/j.foodhyd.2016.11.042
34	T P Wang, L L Chang, S N Chang, E C Wang, L C Hwang, Y H Chen, Y M Wang (2012). Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan. Process Biochemistry, 47(3): 550–554 https://doi.org/10.1016/j.procbio.2011.12.015
35	G Żyła, M Stompor, M Kopańska, R Stagraczyński, J Fal, J Traciak, M Trybus, S Wolski, I Zawlik, J Cebulski, M Konefał-Janocha, M Cholewa (2017). The influence of sonication and silver nanoparticles doped on viscoelastic structure of Agarose Gel. Acta Physica Polonica A, 132(1): 152–154 https://doi.org/10.12693/APhysPolA.132.152

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