Effect of nitrobenzene on the performance and bacterial community in an expanded granular sludge bed reactor treating high-sulfate organic wastewater

doi:10.1007/s11783-019-1090-y

Front. Environ. Sci. Eng.

2019, Vol. 13

Issue (1) : 6 https://doi.org/10.1007/s11783-019-1090-y

RESEARCH ARTICLE

Effect of nitrobenzene on the performance and bacterial community in an expanded granular sludge bed reactor treating high-sulfate organic wastewater

Jun Li^1,², Wentao Li¹, Gan Luo¹, Yan Li¹(

), Aimin Li^1,²

¹. State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China
². Nanjing University & Yancheng Academy of Environmental Protection Technology and Engineering, Yancheng 224000, China

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Abstract

Less than 50 mg/L nitrobenzene brought little effect on anaerobic sulfate reduction.

Kinetics of sulfate reduction under different nitrobenzene contents was studied.

Increased nitrobenzene contents greatly changed the bacterial community structure.

Genus Desulfovibrio played the key role in anaerobic sulfate reduction process.

Nitrobenzene (NB) is frequently found in wastewaters containing sulfate and may affect biological sulfate reduction process, but information is limited on the responses of sulfate reduction efficiency and microbial community to the increased NB contents. In this study, a laboratory-scale expanded granular sludge bed reactor was operated continuously to treat high-sulfate organic wastewater with increased NB contents. Results successfully demonstrated that the presence of more than 50 mg/L NB depressed sulfate reduction and such inhibition was partly reversible. Bath experiments showed that the maximum specific desulfuration activity (SDA) decreased from 135.80 mg SO₄²^?/gVSS/d to 30.78 mg SO₄²^?/gVSS/d when the NB contents increased from none to 400 mg/L. High-throughput sequencing showed that NB also greatly affected bacterial community structure. Bacteroidetes dominated in the bioreactor. The abundance of Proteobacteria increased with NB addition while Firmicutes presented an opposite trend. Proteobacteria gradually replaced Firmicutes for the dominance in response to the increase of influent NB concentrations. The genus Desulfovibrio was the dominant sulfate-reducing bacteria (SRB) with absence or presence of NB, but was inhibited under high content of NB. The results provided better understanding for the biological sulfate reduction under NB stress.

Keywords Nitrobenzene (NB) Sulfate-reducing bacteria (SRB) Bacterial community Sulfate reduction High-throughput sequencing

Corresponding Author(s): Yan Li

Issue Date: 03 December 2018

Cite this article:

Jun Li,Wentao Li,Gan Luo, et al. Effect of nitrobenzene on the performance and bacterial community in an expanded granular sludge bed reactor treating high-sulfate organic wastewater[J]. Front. Environ. Sci. Eng., 2019, 13(1): 6.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-019-1090-y
https://academic.hep.com.cn/fese/EN/Y2019/V13/I1/6

Fig.1 SO₄²^? removal performances of EGSB reactor with different influent NB concentrations.

Fig.2 COD removal performances of EGSB reactor with different influent NB concentrations.

Fig.3 Sulfate removal with different NB concentrations in batch experiments (a) efficiency and (b) first-order kinetics model.

Tab.1 Batch experiments parameters with different NB concentrations

Tab.2 Change of bacteria community biodiversity with increased influent NB concentrations

Fig.4 Taxonomic classification on phylum level in EGSB reactor with different influent NB contents.

Fig.5 Taxonomic classification on genus level in EGSB reactor with different influent NB contents.

Fig.6 NB removal performances of EGSB reactor with different influent NB concentrations.

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