Exogenously applied oxalic acid assists in the phytoremediation of Mn by Polygonum pubescens Blume cultivated in three Mn-contaminated soils

doi:10.1007/s11783-020-1380-4

Front. Environ. Sci. Eng.

2021, Vol. 15

Issue (5) : 86 https://doi.org/10.1007/s11783-020-1380-4

RESEARCH ARTICLE

Exogenously applied oxalic acid assists in the phytoremediation of Mn by Polygonum pubescens Blume cultivated in three Mn-contaminated soils

Kehui Liu^1,², Jie Xu^1,², Chenglong Dai^1,², Chunming Li^1,^2,⁴, Yi Li^1,³(

), Jiangming Ma^1,^2,⁵, Fangming Yu^1,³(

)

¹. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education), Guangxi Normal University, Guilin 541004, China
². College of Life Science, Guangxi Normal University, Guilin 541004, China
³. College of Environment and Resource, Guangxi Normal University, Guilin 541004, China
⁴. School of Life Sciences, Fudan University, Shanghai 200438, China
⁵. Institute of Sustainable Development and Innovation, Guangxi Normal University, Guilin 541006, China

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Abstract

• The OA supply significantly increased the water-extractable Mn in all soils.

• All OA supply levels promoted plant growth in unexplored soil.

• Low OA supply level promoted plant growth in explored and tailing soils.

• OA amendment increased the Mn concentrations and total Mn in P. pubescens.

• P. pubescens experienced less Mn stress in unexplored soil than in the other two soils.

The current study evaluated the effects of oxalic acid (OA) application on the growth and Mn phytoremediation efficiency of Polygonum pubescens Blume cultivated in three different manganese (Mn)-contaminated soils sampled from an unexplored area (US), an explored area (ES) and a tailing area (TS) of the Ertang Mn mine, South China. The supplied levels of OA were 0 (control), 1 (low level), 3 (medium level), and 9 (high level) mmol/kg, referred to as CK, OA1, OA3 and OA9, respectively. The results revealed that the average water-extractable Mn concentrations US, ES and TS amended with OA increased by 214.13, 363.77 and 266.85%, respectively. All OA supply levels increased plant growth and Mn concentrations in US. The low OA supply level increased plant growth in ES and TS; however, contrasting results were found for the medium and high OA supply levels. Plant Mn concentrations and total Mn increased in ES and TS in response to all OA supply levels. Total Mn in the aerial parts increased by 81.18, 44.17 and 83.17% in US, ES and TS, respectively; the corresponding percentages for the whole plants were 81.53, 108.98 and 77.91%, respectively. The rate of ·O₂⁻ production and malondialdehyde (MDA) concentrations increased in response to OA amendment, especially the medium and high OA supply levels in ES and TS. In general, antioxidant enzymes might play a vital role in alleviating Mn stress in plants cultivated in US, while non-enzymatic antioxidants might be the main factor for plants cultivated in ES and TS.

Keywords Phytoremediation Mn-contaminated soil Mn hyperaccumulator Oxalic acid Remediation efficiency

Corresponding Author(s): Yi Li,Fangming Yu

Issue Date: 17 December 2020

Cite this article:

Kehui Liu,Jie Xu,Chenglong Dai, et al. Exogenously applied oxalic acid assists in the phytoremediation of Mn by Polygonum pubescens Blume cultivated in three Mn-contaminated soils[J]. Front. Environ. Sci. Eng., 2021, 15(5): 86.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1380-4
https://academic.hep.com.cn/fese/EN/Y2021/V15/I5/86

Fig.1 Water-extractable Mn concentration in three Mn-contaminated soils in response to OA amendment.

Fig.2 Changes in Mn concentrations (g/kg) and accumulation (g/plant) in P. pubescens cultivated in three Mn-contaminated soils amended with OA.

Fig.3 Changes in BF and TF values of Mn in response to OA amendment. (1) The BFs for the roots, stems and leaves represent the Mn concentration in each organ relative to that in the soil. (2) The TFs for S/R, L/R and L/S represent the Mn concentration in the stems relative to that in the roots, the Mn concentration in the leaves relative to that in the roots, and the Mn concentration in the leaves relative to that in the stems, respectively.

Fig.4 Changes in plant height (cm/plant), root length (cm/plant) and plant biomass (g/plant, dry weight) of P. pubescens cultivated in three Mn-contaminated soils amended with OA.

Fig.5 Changes in photosynthetic pigments in P. pubescens cultivated in three Mn-contaminated soils amended with OA. Chla, Chlb, the Chla/Chlb and Cars represent chlorophyll a, chlorophyll b, the ratio of chlorophyll a to chlorophyll b and carotenoids, respectively.

Tab.1 Changes in H₂O₂, ·O₂^.^? and MDA concentrations of P. pubescens cultivated in three different Mn-contaminated soils amended with OA

Fig.6 Changes in SOD, POD, CAT and APX activity in P. pubescens cultivated in three different Mn-contaminated soils in response to OA amendment. SOD, POD, CAT and APX represent superoxide dismutase, peroxidase, catalase and ascorbate peroxidase, respectively.

Tab.2 Changes in GSH, PCs and -SH of P. pubescens cultivated in three different Mn-contaminated soils amended with OA

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