The development of graphene-based devices for cell biology research

doi:10.1007/s11706-014-0228-x

Front. Mater. Sci.

2014, Vol. 8

Issue (2) : 107-122 https://doi.org/10.1007/s11706-014-0228-x

REVIEW ARTICLE

The development of graphene-based devices for cell biology research

Zhi-Qin YAN¹,Wei ZHANG^2,^*(

)

1. School of Mechanical, Jinzhong University, Yuci, Jinzhong 030600, China
2. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, Zhongguancun, Beijing 100190, China

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Abstract

Graphene has emerged as a new carbon nanoform with great potential in many applications due to its exceptional physical and chemical properties. Especially, graphene and its derivatives are also gaining a lot of interest in the biomedical field as new components for biosensors, tissue engineering, and drug delivery. This review presents unique properties of graphene, the bio-effects of graphene and its derivatives, especially their interactions with cells and the development of graphene-based biosensors and nanomedicines for cancer diagnosis and treatment.

Keywords graphene graphene oxide (GO) cell sensor cancer

Corresponding Author(s): Wei ZHANG

Issue Date: 24 June 2014

Cite this article:

Zhi-Qin YAN,Wei ZHANG. The development of graphene-based devices for cell biology research[J]. Front. Mater. Sci., 2014, 8(2): 107-122.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-014-0228-x
https://academic.hep.com.cn/foms/EN/Y2014/V8/I2/107

Fig.1 SGS internalization within Hep3B cancer cells. (a)(b)(c)(d)(e)(f) Transmission electron microscopy (TEM) images of internalized carbonaceous material and SGSs within Hep3B liver cancer cells. (Reproduced with permission from Ref. [28]).

Fig.2 (a) The differentiation of NSCs on 3D-glial fibrillary (GF) scaffold. Left, Western blot analysis of nestin, Tuj1, glial fibrillary acidic protein (GFAP) and receptor-interacting protein (RIP) protein expression of differentiated NSCs on 2D graphene films and 3D-GFs; Right, relative optical densities of nestin, Tuj1, GFAP and RIP bands. (Reproduced with permission from Ref. [39]). (b) Fluorescence images of hNSCs differentiated on glass (left) and graphene (right) after one month differentiation. The differentiated hNSCs were immunostained with GFAP (red) for astroglial cells, class III β-tubulin (TUJ1) (green) for neural cells, and 4′,6-diamidino-2-phenylindole (DAPI) (blue) for nuclei. Note that more hNSCs were adhered to graphene than to glass. Scale bars= 200 μm. (Reproduced with permission from Ref. [41]).

Fig.3 GO chip and functionalization/characterization of GO. (a) Schematic of the GO chip. (b) Schematic showing the conjugation chemistry between functionalized GO nanosheets and EpCAM antibodies. GO nanosheets are adsorbed onto the gold pattern. The N-(g-maleimidobutyryloxy) succinimide ester (GMBS) cross-linker binds to PL-PEG-NH₂ on the GO nanosheets. The NeutrAvidin is connected to the GMBS and biotinylated EpCAM. (c) Preparation procedures for the functionalized GO. (Reproduced with permission from Ref. [57]).

Fig.4 Top, fluorescence images of the MCF-7/ADR cells after the incubation with GQDs, DOX and DOX/GQDs: (a) bright-field, (b) nuclei stained by Hoechst, (c) fluorescence of DOX excited at 510 nm, and (d) overlays of (a), (b), and (c). Scale bars= 50 μm. Bottom, cell viability of the MCF-7/ADR cells exposed to different concentrations of DOX alone (dark bars) and DOX with GQDs for 24 h (grey bars). The control samples are the untreated cells and the cells with GQDs only (gray bar). (Reproduced with permission from Ref. [<CitationRef CitationID="cit68"></CitationRef>]).

Fig.5 (a) Schematic illustration of the synthetic procedure for UCNPs-NGO: Numbers of core-shell structured UCNPs being covalently grafted with NGO via bifunctional polyethylene glycol. (b) Schematic illustration of UCNPs-NGO/ZnPc as a multifunctional theranostic nanoplatform for cancer treatment. (Reproduced with permission from Ref. [73]).

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