Remineralization of initial enamel caries <i>in vitro</i> using a novel peptide based on amelogenin

doi:10.1007/s11706-015-0298-4

Front. Mater. Sci.

2015, Vol. 9

Issue (3) : 293-302 https://doi.org/10.1007/s11706-015-0298-4

RESEARCH ARTICLE

Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin

Danxue LI,Xueping LV,Huanxin TU,Xuedong ZHOU,Haiyang YU,Linglin ZHANG(

)

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

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Abstract

Dental caries is the most common oral disease with high incidence, widely spread and can seriously affect the health of oral cavity and the whole body. Current caries prevention measures such as fluoride treatment, antimicrobial agents, and traditional Chinese herbal, have limitations to some extent. Here we design and synthesize a novel peptide based on the amelogenin, and assess its ability to promote the remineralization of initial enamel caries lesions. We used enamel blocks to form initial lesions, and then subjected to 12-day pH cycling in the presence of peptide, NaF and HEPES buffer. Enamel treated with peptide or NaF had shallower, narrower lesions, thicker remineralized surfaces and less mineral loss than enamel treated with HEPES. This peptide can promote the remineralization of initial enamel caries and inhibit the progress of caries. It is a promising anti-caries agent with various research prospects and practical application value.

Keywords dental caries peptide remineralization

Corresponding Author(s): Linglin ZHANG

Online First Date: 28 May 2015 Issue Date: 23 July 2015

Cite this article:

Danxue LI,Xueping LV,Huanxin TU, et al. Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin[J]. Front. Mater. Sci., 2015, 9(3): 293-302.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-015-0298-4
https://academic.hep.com.cn/foms/EN/Y2015/V9/I3/293

Tab.1 The pH-cycling model in the experiment

Fig.1 X-ray photoelectron spectroscopy of peptide adsorbed to demineralized enamel: the demineralized enamel treated with peptide (a); the demineralized enamel treated with buffer only (b).

Fig.2 CD spectrum of the peptide.

Fig.3 SEM images of enamels before and after pH cycling: (a) demineralized; (b) NaF; (c) peptide; (d) HEPES.

Fig.4 PLM images of enamel sections from all treatment groups before and after pH cycling: (a) peptide; (b) NaF; (c) HEPES.

Fig.5 TRM images of all treatment groups after pH-cycling: (a) peptide; (b) NaF; (c) HEPES.

Tab.2 Mineral loss, lesion depth of all groups before and after pH-cycling (mean±SD, N = 10)

Tab.3 Mineral content of all groups at several selected depths (mean±SD, N = 10)

Tab.1

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