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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

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Front. Mater. Sci.    2015, Vol. 9 Issue (3) : 272-281    https://doi.org/10.1007/s11706-015-0303-y
RESEARCH ARTICLE
Development of part-dissolvable chitosan fibers with surface N-succinylation for wound care dressing
Guohui SUN1,Chao FENG1,Ming KONG1,Xiaojie CHENG1,Jiaojiao BING1,Guixue XIA1,Zixian BAO1,Hyunjin PARK2,*(),Xiguang CHEN1,*()
1. College of Marine Life Science, Ocean University of China, Qingdao 266003, China
2. College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea
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Abstract

To enhance the liquor absorptivity of chitosan fibers (CS-Fs), N-succinyl surface-modified chitosan fibers (NSCS-Fs) were developed and evaluated for wound healing. The NSCS-Fs exhibited cracks on the surface and high liquor absorbing capacity with absorbing--dissolvable equilibrium state in phosphate buffer solution (PBS). The bacteriostasis ratios of NSCS-Fs against E. coli, S. aureus and C. albicans were higher than 80%. No cytotoxicity has been found for mouse embryo fibroblasts (MEFs) treated with NSCS-Fs leach liquor. Acute oral toxicity and skin irritation experiment were taken to evaluate the safety of NSCS-Fs in vitro. Muscle implant study showed that NSCS-Fs were biodegradable and non-toxic in vivo. These results suggested that the surface modified NSCS-Fs had favorable biological properties and improved liquor absorptivity, indicating that they could be used as promising dressing materials for wound care.
Keywords chitosan fiber      surface N-succinylation      liquor absorptivity      part-dissolvability      wound dressing     
Corresponding Author(s): Hyunjin PARK,Xiguang CHEN   
Online First Date: 16 June 2015    Issue Date: 23 July 2015
 Cite this article:   
Guohui SUN,Chao FENG,Ming KONG, et al. Development of part-dissolvable chitosan fibers with surface N-succinylation for wound care dressing[J]. Front. Mater. Sci., 2015, 9(3): 272-281.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-015-0303-y
https://academic.hep.com.cn/foms/EN/Y2015/V9/I3/272
Fig.1  Synthesis of the NSCS-Fs with succinic anhydride.
Fig.2  (a) FTIR spectra. (b)13C NMR spectra of CS-Fs and NSCS-Fs. (c) SEM images of the three CS materials with different shapes: CS (×18) (i); CS-Fs (×500) (ii); NSCS-Fs (×500) (iii).
Sample Elemental analysis DS /% MW /(g?mol-1)
f(C) /% f(N) /% f(C)/f(N)
CS 45.81 8.8 5.21 – 9.05×105
CS-Fs 40.50 7.89 5.13 – 9.05×105
NSCS-Fs 41.94 7.60 5.52 21.77 9.79×105
Tab.1  The properties of CS, CS-Fs, and NSCS-Fs
Fig.3  Schematic illustrations of the physical structure of NSCS-Fs and the swelling process: (a) the structure of NSCS-Fs bundles; (b) the absorbing process of NSCS-Fs in PBS; (c) NSCS-Fs dissolved in PBS; (d) achieved the absorbing–dissolvable equilibrium state of NSCS-Fs in solution; (e) liquid absorption kinetics and the corresponding images of CS, CS-Fs and NSCS-Fs in PBS (n = 3).
Fig.4  Comparison of the inhibition ratios of CS, CS-Fs and NSCS-Fs against E. coli, S. aureus and C. albicans. In this study, CS was just against E. coli. “*”: p<0.05 (n = 5).
Fig.5  RGR of MEF cells in the culture media after addition of leach liquors detected by MTT assay in vitro (n = 5). The control was just added with culture medium and the viability was set as 100%: (a) CS; (b) CS-Fs; (c) NSCS-Fs.
Group Sex wo /g wf /g wa /g wad /g
CS group Female 18.80±0.56 28.60±1.75 9.80±1.81 0.70±0.13
Male 19.60±2.23 37.12±2.37 17.50±1.77 1.25±0.13
CS-Fs group Female 19.32±1.58 29.60±2.10 10.28±2.24 0.74±0.16
Male 18.80±1.13 36.92±2.32 18.12±2.58 1.29±0.18
NSCS-Fs group Female 17.98±1.91 30.06±3.36 12.08±3.15 0.86±0.22
Male 18.84±1.34 33.24±2.47 14.40±3.47 1.03±0.25
Control group Female 18.90±1.30 30.02±1.26 11.12±1.38 0.79±0.10
Male 19.82±0.99 35.70±2.40 15.88±3.30 1.13±0.23
Tab.2  The data of weight change between the experimental groups and the control group (mean±SD, n = 5)
Fig.6  Light microscopy images of biodegradability after implantation of CS, CS-Fs and NSCS-Fs for 4 weeks (×400), respectively. The control just had a surgery without any insets administration. Arrows indicate that macrophages swallowed the small molecules.
C. albicansCandida albicans
CSchitosan
CS-Fchitosan fiber
DDdegree of deacetylation
DMEMDulbecco’s modified Eagle’s medium
DSdegree of substitution
E. coliEscherichia coli
FTIRFourier transform infrared spectroscopy
GPCgel permeation chromatography
LARliquor absorbing ratio
MEFmouse embryo fibroblast
MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
MWmolecular weight
NMRnuclear magnetic resonance
NSCS-FN-succinyl surface modified chitosan fiber
PBSphosphate buffer solution
PUpolyurethane
RGRrelative growth rate
RIGratio of inhibited growth of bacteria
S. aureusStaphylococcus aureus
SEMscanning electron microscopy
SIRSsystemic inflammatory response syndrome
SPFspecific pathogen free
Tab.1  
C. albicansCandida albicans
CSchitosan
CS-Fchitosan fiber
DDdegree of deacetylation
DMEMDulbecco’s modified Eagle’s medium
DSdegree of substitution
E. coliEscherichia coli
FTIRFourier transform infrared spectroscopy
GPCgel permeation chromatography
IRGratio of inhibited the growth of bacteria
LARliquor absorbing ratio
MEFmouse embryo fibroblast
MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
MWmolecular weight
NMRnuclear magnetic resonance
NSCS-FN-succinyl surface modified chitosan fiber
PBSphosphate buffer solution
PUpolyurethane
RGRrelative growth rate
S. aureusStaphylococcus aureus
SEMscanning electron microscopy
SIRSsystemic inflammatory response syndrome
SPFspecific pathogen free
Tab.1  
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