Upconversion luminescence Ca--Mg--Si bioactive glasses synthesized using the containerless processing technique

doi:10.1007/s11706-019-0484-x

Front. Mater. Sci.

2019, Vol. 13

Issue (4) : 399-409 https://doi.org/10.1007/s11706-019-0484-x

RESEARCH ARTICLE

Upconversion luminescence Ca--Mg--Si bioactive glasses synthesized using the containerless processing technique

Qin LI¹, Min XING¹, Lan CHANG^1,², Linlin MA¹, Zhi CHEN³, Jianrong QIU³, Jianding YU¹(

), Jiang CHANG¹(

)

¹. State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China
². School of Pharmacy, Fudan University, Shanghai 201203, China
³. State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

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Abstract

In this study, a series of Er³⁺/Yb³⁺ co-doped Ca--Mg--Si glasses were prepared via the containerless processing. Phase composition and luminescent properties of the prepared materials were investigated through XRD and spectrometry, and bioactivity, biocompatibility and cytotoxicity were evaluated. The XRD patterns indicated that akermanite (AKT) ceramic powders were completely transformed into the glassy phase (AKT-G, EYA) through the containerless processing, which exhibit upconversion luminescence, and the luminescence intensity increased with the increase of the doping amount of Er³⁺ and Yb³⁺. High amount of Yb³⁺ doping and existence of Ca²⁺ in glasses resulted in more intensive red-light emission. The SEM observation, combined with EDS analysis, and cell culture experiments showed that the as-prepared glasses were nontoxic, biocompatible and bioactive. All these results demonstrated that the contai-nerless processing is a facile method for preparing homogeneous luminescent bioactive glasses. Furthermore, this luminescent Ca--Mg--Si glasses may be used as bone implant materials to study the in vivo distribution of degradation products of bone implants, which may be of great significance for the development and clinical application of new bone grafting materials.

Keywords containerless processing akermanite Er³⁺/Yb³⁺ codoped Ca--Mg--Si glass upconversion luminiscence bioactivity

Corresponding Author(s): Jianding YU,Jiang CHANG

Online First Date: 27 November 2019 Issue Date: 04 December 2019

Cite this article:

Qin LI,Min XING,Lan CHANG, et al. Upconversion luminescence Ca--Mg--Si bioactive glasses synthesized using the containerless processing technique[J]. Front. Mater. Sci., 2019, 13(4): 399-409.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-019-0484-x
https://academic.hep.com.cn/foms/EN/Y2019/V13/I4/399

Tab.1 Nominal compositions of Er³⁺/Yb³⁺ co-doped Ca–Mg–Si glasses (after conversion to molar ratio)

Fig.1 Ca–Mg–Si glasses prepared by the containerless processing: (a) AKT-G; (b) EYA-1; (c) EYA-2; (d) EYA-3; (e) EYA-4.

Fig.2 XRD patterns of as-prepared samples AKT-G, EYA-1, EYA-2, EYA-3 and EYA-4. The inset illustrates the XRD pattern of AKT-P raw material.

Fig.3 Luminescence photos of as-prepared glasses excited by 980 nm laser: (a) EYA-1; (b) EYA-2; (c) EYA-3; (d) EYA-4.

Fig.4 Upconversion luminescence spectra of Er³⁺/Yb³⁺ co-doped bioactive glasses: EYA-1, EYA-2, EYA-3 and EYA-4.

Fig.5 Relationship between the upconversion luminescence and the Er³⁺/Yb³⁺ doping concentration under the same power 0.95 A.

Fig.6 Morphologies of as-prepared (a) EYA-2 before soaking in SBF, and (b) EYA-1, (c) EYA-2, (d) EYA-3 and (e) EYA-4 after soaking in SBF for 14 d.

Fig.7 EDS results of as-prepared (a) EYA-2 before soaking in SBF, and (b) EYA-1, (c) EYA-2, (d) EYA-3 and (e) EYA-4 after soaking in SBF for 14 d.

Fig.8 (a) The proliferation of HUVECs in the ionic extracts of glasses after culturing for 5 d. The cells cultured in medium without ionic extracts of glasses were treated as the control group. (b) The proliferation of HDFs in the ionic extracts of glasses after culturing for 7 d. The cells cultured in medium without ionic extracts of glasses were treated as the control group. (*p<0.05)

Fig.9 Expression of angiogenesis related genes including (a) VEGF and (b) KDR in HUVECs cultured with ionic extracts of glasses after 3 d. Expression of angiogenesis related genes including (c) VEGF and (d) bFGF in HDFs cultured with ionic extracts of glasses after 3 d. The cells cultured in medium without ionic extracts of glasses were treated as the control group. (*p<0.05)

Fig.10 (a) The cell migration of HDFs in ionic extracts of glasses after scratching (0 h) and migration for 26 h. (b) The migration ratios of HDFs cultured with ionic extracts of glasses. The cells cultured in medium without ionic extracts of glasses were treated as the control group. (*p<0.05)

Fig.11 (a) The cell migration of HUVECs in ionic extracts of glasses after scratching (0 h) and migration for 26 h. (b) The migration ratios of HUVECs cultured with ionic extracts of glasses. The cells cultured in medium without ionic extracts of glasses were treated as the control group. (*p<0.05)

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