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Engineering human pluripotent stem cell-derived 3D brain tissues for drug discovery |
| Ha Nam Nguyen,Hongjun Song,Guoli Ming |
| 1. Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
2. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
3. The Solomon H Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
4. Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA |
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Abstract Abstract The quest to find novel therapeutics for mental and neurological disorders has been hindered by the lack of access to live human brain samples and relevant experimental models. Conventional 2D human pluripotent stem cell-derived neuronal cultures and animal models do not fully recapitulate many endogenous human biochemical processes and diseasephenotypes. Currently, the majority of candidate drugs obtained from preclinical testing in conventional systems
does not usually translate into success and have a high failure rate in clinical trials. Recent advancements in bioengineering and stem cell technologies have resulted in three-dimensional brain-like tissues, such as oragnoids,which better resemble endogenous tissue and are more
physiologically relevant than monolayer cultures. These brain-like tissues can bridge the gap between existing models and the patient, and may revolutionize the field of translational neuroscience. Here, we discuss utilities and challenges of using stem cell-derived human brain
tissues in basic research and pharmacotherapy
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| Keywords
stem cell-derived brain tissues, three-threedimensional
(3D), translational neuroscience, brain organoids
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Issue Date: 18 December 2016
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