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Prot Cell    2010, Vol. 1 Issue (11) : 987-998    https://doi.org/10.1007/s13238-010-0120-0      PMID: 21153516
REVIEW
Role of tegument proteins in herpesvirus assembly and egress
Haitao Guo1,2, Sheng Shen1,2, Lili Wang1,2, Hongyu Deng1()
1. CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100080, China
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Abstract

Morphogenesis and maturation of viral particles is an essential step of viral replication. An infectious herpesviral particle has a multilayered architecture, and contains a large DNA genome, a capsid shell, a tegument and an envelope spiked with glycoproteins. Unique to herpesviruses, tegument is a structure that occupies the space between the nucleocapsid and the envelope and contains many virus encoded proteins called tegument proteins. Historically the tegument has been described as an amorphous structure, but increasing evidence supports the notion that there is an ordered addition of tegument during virion assembly, which is consistent with the important roles of tegument proteins in the assembly and egress of herpesviral particles. In this review we first give an overview of the herpesvirus assembly and egress process. We then discuss the roles of selected tegument proteins in each step of the process, i.e., primary envelopment, deenvelopment, secondary envelopment and transport of viral particles. We also suggest key issues that should be addressed in the near future.
Keywords herpesvirus      tegument      assembly      egress      transport     
Corresponding Author(s): Deng Hongyu,Email:rrain6@yahoo.com   
Issue Date: 01 November 2010
 Cite this article:   
Sheng Shen,Lili Wang,Hongyu Deng, et al. Role of tegument proteins in herpesvirus assembly and egress[J]. Prot Cell, 2010, 1(11): 987-998.
 URL:  
https://academic.hep.com.cn/pac/EN/10.1007/s13238-010-0120-0
https://academic.hep.com.cn/pac/EN/Y2010/V1/I11/987
Fig.1  
The virion particle is approximately 200 nm in diameter. A linear double-stranded DNA genome is packaged within an icosahedral capsid to form the nucleocapsid. The capsid is embedded in a matrix known as the tegument layer, which contains many virus coded proteins. The tegument is itself surrounded by the envelope, a lipid membrane containing several viral glycoproteins.
Fig.1  
The virion particle is approximately 200 nm in diameter. A linear double-stranded DNA genome is packaged within an icosahedral capsid to form the nucleocapsid. The capsid is embedded in a matrix known as the tegument layer, which contains many virus coded proteins. The tegument is itself surrounded by the envelope, a lipid membrane containing several viral glycoproteins.
HSV-1 tegu- ment proteinsnomenclature of HSV-1 tegument homologs
HCMVEBVKSHV
UL7UL103BBRF2ORF42
UL11UL99BBLF1ORF38
UL13UL97BGLF4ORF36
UL14UL95BGLF3ORF34
UL16UL94BGLF2ORF33
UL21UL87BcRF1ORF24
UL23NABXLF2ORF21
UL36UL48BPLF1ORF64
UL37UL47BOLF1ORF63
UL51UL71BSRF1ORF55
UL41NANANA
UL46NANANA
UL47NANANA
UL48NANANA
UL49NANANA
UL50NANANA
UL55NANANA
US2NANANA
US3NANANA
US10NANANA
US11NANANA
RL1 (ICP34.5)NANANA
RL2 (ICP0)NANANA
RS1 (ICP4)NANANA
Tab.1  Conservation of herpesvirus tegument proteins
Fig.2  
Viral genomes are packaged into preformed capsids in the nucleus. Through primary envelopment at the inner nuclear membrane and deenvelopment at the outer nuclear membrane, the nucleocapsid is transported into the cytoplasm from the nucleus. Acquisition of tegument proteins onto the nucleocapsid is completed in the cytoplasm. The immature virion is transported via the microtubules into a trans-Golgi derived vesicle containing viral glycoproteins. After transport of the vesicle to the cell surface, the vesicle and plasma membrane fuse, resulting in the egress of a mature, enveloped virion.
Fig.2  
Viral genomes are packaged into preformed capsids in the nucleus. Through primary envelopment at the inner nuclear membrane and deenvelopment at the outer nuclear membrane, the nucleocapsid is transported into the cytoplasm from the nucleus. Acquisition of tegument proteins onto the nucleocapsid is completed in the cytoplasm. The immature virion is transported via the microtubules into a trans-Golgi derived vesicle containing viral glycoproteins. After transport of the vesicle to the cell surface, the vesicle and plasma membrane fuse, resulting in the egress of a mature, enveloped virion.
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