Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding <italic>in vitro

doi:10.1007/s13238-012-2006-9

Prot Cell

2012, Vol. 3

Issue (2) : 153-159 https://doi.org/10.1007/s13238-012-2006-9 PMID: 22322890

RESEARCH ARTICLE

Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro

Juan Li, Bin Liu, Xiaofei Gao, Zhixing Ma, Tianyi CaoSong, Yan-ai Mei, Yufang Zheng(

)

School of Life Sciences, Fudan University, Shanghai 200433, China

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Abstract

The sigma-1 receptor is a molecular chaperone protein highly enriched in the brain. Recent studies linked it to many diseases, such as drug addition, Alzheimer’s disease, stroke, depression, and even cancer. Sigma-1 receptor is enriched in lipid rafts, which are membrane microdomains essential in signaling processes. One of those signaling processes is ADAM17- and ADAM10-dependent ectodomain shedding. By using an alkaline phosphatase tagged substrate reporter system, we have shown that ADAM10-dependent BTC shedding was very sensitive to both membrane lipid component change and sigma-1 receptor agonist DHEAS treatment while ADAM17-dependent HB-EGF shedding was not; and overexpression of sigma-1 receptor diminished ADAM17- and ADAM10-dependent shedding. Our results indicate that sigma-1 receptor plays an important role in modifying the function of transmembrane proteases.

Keywords sigma-1 receptor ADAM17 ADAM10 shedding lipid raft

Corresponding Author(s): Zheng Yufang,Email:zhengyf@fudan.edu.cn

Issue Date: 01 February 2012

Cite this article:

Juan Li,Bin Liu,Xiaofei Gao, et al. Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro[J]. Prot Cell, 2012, 3(2): 153-159.

URL:

https://academic.hep.com.cn/pac/EN/10.1007/s13238-012-2006-9
https://academic.hep.com.cn/pac/EN/Y2012/V3/I2/153

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