Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media

doi:10.1007/s11705-018-1766-z

Front. Chem. Sci. Eng.

2019, Vol. 13

Issue (1) : 90-97 https://doi.org/10.1007/s11705-018-1766-z

RESEARCH ARTICLE

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media

Vo-Van Giau¹, Yoon-Hee Park², Kyu-Hwan Shim¹, Sang-Wook Son²(

), Seong-Soo A. An¹(

)

¹. Department of Bionanotechnology and Gachon Medical Research Institute, Gachon University and Gachon Medical Research Institute, Seongnam, Korea
². Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea

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Abstract

The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomaterial’s surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography–mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.

Keywords ZnONPs nanoparticles protein corona ClueGO

Corresponding Author(s): Sang-Wook Son,Seong-Soo A. An

Just Accepted Date: 27 July 2018 Online First Date: 25 January 2019 Issue Date: 25 February 2019

Cite this article:

Vo-Van Giau,Yoon-Hee Park,Kyu-Hwan Shim, et al. Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media[J]. Front. Chem. Sci. Eng., 2019, 13(1): 90-97.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-018-1766-z
https://academic.hep.com.cn/fcse/EN/Y2019/V13/I1/90

Tab.1 Diversity of the abundant common proteins absorbed to ZnONPs between 5 and 60 min

Fig.1 ZnO nanoparticle (ZnONP) geometric diameter measured using transmission electron microscopy (TEM). (A) TEM images of ZnONP without incubation; (B and C) TEM image of ZnONP incubated in cell culture medium at 37°C for 5 and 60 min respectively

Tab.2 List of the abundant specific proteins identified on ZnONPs at 5 and 60 min

Fig.2 Visualized biological processes associated with binding of proteins from culture media with ZnO nanoparticles after (A) 5 min and (B) 60 min

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