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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2019, Vol. 13 Issue (6) : 85    https://doi.org/10.1007/s11783-019-1169-5
RESEARCH ARTICLE
Effects of Al3+ on pollutant removal and extracellular polymeric substances (EPS) under anaerobic, anoxic and oxic conditions
Lanhe Zhang1,2, Jing Zheng1, Jingbo Guo3(), Xiaohui Guan1, Suiyi Zhu4, Yanping Jia1, Jian Zhang1, Xiaoyu Zhang2, Haifeng Zhang1
1. School of Chemical Engineering, Northeast Electric Power University, Jilin 132012, China
2. Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun 130118, China
3. School of Civil and Architecture Engineering, Northeast Electric Power University, Jilin 132012, China
4. School of Environment, Northeast Normal University, Changchun 130117, China
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Abstract

The highest removal efficiencies of COD and TN were achieved under 10 mg/L of Al3+.

The highest TP removal efficiency occurred under 30 mg/L of Al3+.

EPS, PS and PN concentrations increased with the addition of Al3+.

Sludge properties significantly changed with the addition of Al3+.

Aluminum ions produced by aluminum mining, electrolytic industry and aluminum-based coagulants can enter wastewater treatment plants and interact with activated sludge. They can subsequently contribute to the removal of suspended solids and affect activated sludge flocculation, as well as nitrogen and phosphorus removal. In this study, the effects of Al3+ on pollutant removal, sludge flocculation and the composition and structure of extracellular polymeric substances (EPS) were investigated under anaerobic, anoxic and oxic conditions. Results demonstrated that the highest chemical oxygen demand (COD) and total nitrogen (TN) removal efficiencies were detected for an Al3+ concentration of 10 mg/L. In addition, the maximal dehydrogenase activity and sludge flocculation were also observed at this level of Al3+. The highest removal efficiency of total phosphorus (TP) was achieved at an Al3+ concentration of 30 mg/L. The flocculability of sludge in the anoxic zone was consistently higher than that in the anaerobic and oxic zones. The addition of Al3+ promoted the secretion of EPS. Tryptophan-like fluorescence peaks were detected in each EPS layer in the absence of Al3+. At the Al3+ concentration of 10 mg/L, fulvic acid and tryptophan fluorescence peaks began to appear, while the majority of protein species and the highest microbial activity were also detected. Low Al3+ concentrations (<10 mg/L) could promote the removal efficiencies of COD and TN, yet excessive Al3+ levels (>10 mg/L) weakened microbial activity. Higher Al3+ concentrations (>30 mg/L) also inhibited the release of phosphorus in the anaerobic zone by reacting with PO43-.

Keywords Extracellular polymeric substances      Activated sludge      Aluminum ion      A2O      Wastewater     
Corresponding Authors: Jingbo Guo   
Issue Date: 19 November 2019
 Cite this article:   
Lanhe Zhang,Jing Zheng,Jingbo Guo, et al. Effects of Al3+ on pollutant removal and extracellular polymeric substances (EPS) under anaerobic, anoxic and oxic conditions[J]. Front. Environ. Sci. Eng., 2019, 13(6): 85.
 URL:  
http://academic.hep.com.cn/fese/EN/10.1007/s11783-019-1169-5
http://academic.hep.com.cn/fese/EN/Y2019/V13/I6/85
Fig.1  Schematic diagram of the A2O process.
Index Unit Value
COD mg/L 495–505
TN mg/L 65–71
NH4+-N mg/L 52–56
TP mg/L 7.5–8.0
pH 7.4–7.8
T 19–25
Tab.1  Influent wastewater specifications
Fig.2  Effects of Al3+ on COD, TN and TP removal efficiencies in the anaerobic, anoxic and oxic zones. a1, a2 and a3 represent COD, TN and TP changes in the anaerobic zone; b1, b2 and b3 represent COD, TN and TP changes in the anoxic zone; and c1, c2 and c3 represent COD, TN and TP changes in the oxic zone.
Fig.3  Effects of Al3+ on the dehydrogenase content in the different zones of the A2O process.
Fig.4  Effect of Al3+ on the sludge flocculation in the anaerobic, anoxic and oxic zones.
Fig.5  Effects of Al3+ on sludge particle size and Zeta potential in the anaerobic, anoxic and oxic zones.
Fig.6  SEM analysis of activated sludge in different zones under varying Al3+ concentrations. (a), (b) and (c) (including 1–4) represent the anaerobic, anoxic and oxic zones, respectively, while 1–4 represent the Al3+ concentrations of 0, 10, 30 and 40 mg/L, respectively.
Fig.7  Effects of Al3+ on EPS, PN and PS in different zones of the A2O process.
Fig.8  3D fluorescence spectra of LB-EPS under varying concentrations of Al3+ in different zones of the A2O process. (a), (b) and (c) (including 1–4) represent the anaerobic, anoxic and oxic zones, respectively.
1 V Agridiotis, C F Forster, C Carliell-Marquet (2007). Addition of Al and Fe salts during treatment of paper mill effluents to improve activated sludge settlement characteristics. Bioresource Technology, 98(15): 2926–2934
https://doi.org/10.1016/j.biortech.2006.10.004 pmid: 17113285
2 A Baker, M Curry (2004). Fluorescence of leachates from three contrasting landfills. Water Research, 38(10): 2605–2613
https://doi.org/10.1016/j.watres.2004.02.027 pmid: 15159164
3 W Chen, P Westerhoff, J A Leenheer, K Booksh (2003). Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science & Technology, 37(24): 5701–5710
https://doi.org/10.1021/es034354c pmid: 14717183
4 B Frolund, T Griebe, P H Nielsen (1995). Enzymatic activity in the activated-sludge floc matrix. Applied Microbiology and Biotechnology, 43(4): 755–761
https://doi.org/10.1007/s002530050481 pmid: 7546613
5 J Ge, X Meng, Y Song, A Terracciano (2018). Effect of phosphate releasing in activated sludge on phosphorus removal from municipal wastewater. Journal of Environmental Sciences-China, 67(5): 216–223
https://doi.org/10.1016/j.jes.2017.09.004 pmid: 29778155
6 N Hudson, A Baker, D Ward, D M Reynolds, C Brunsdon, C Carliell-Marquet, S Browning (2008). Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England. Science of the Total Environment, 391(1): 149–158
https://doi.org/10.1016/j.scitotenv.2007.10.054 pmid: 18054993
7 K L Hwang, C H Bang, K D Zoh (2016). Characteristics of methane and nitrous oxide emissions from the wastewater treatment plant. Bioresource Technology, 21: 881–884
https://doi.org/10.1016/j.biortech.2016.05.047 pmid: 27237575
8 B Ji, K Yang, H Wang (2015). Impacts of poly-aluminum chloride addition on activated sludge and the treatment efficiency of SBR. Desalination and Water Treatment, 54(9): 2376–2381
https://doi.org/10.1080/19443994.2014.989920
9 A Li, C Zhou, Z L Liu, X Y Xu, Y Zhou, D D Zhou, Y N Tang, F Ma, B E Rittmann (2018). Direct solid-state evidence of H2-induced partial U(VI) reduction concomitant with adsorption by extracellular polymeric substances (EPS). Biotechnology and Bioengineering, 115(7): 1685–1693
https://doi.org/10.1002/bit.26592 pmid: 29574765
10 H J Li, Y Taniguchi (2019). Load-carrying capacity of semi-rigid double-layer grid structures with initial crookedness of member. Engineering Structures, 184(1): 421–433
https://doi.org/10.1016/j.engstruct.2019.01.094
11 M Li, Y G Chen, Y L Su, R Wan, X Zheng (2016). Effect of fulvic acids with different characteristics on biological denitrification. RSC Advances, 6(18): 14993–15001
https://doi.org/10.1039/C5RA26885K
12 X Y Li, S F Yang (2007). Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge. Water Research, 41(5): 1022–1030
https://doi.org/10.1016/j.watres.2006.06.037 pmid: 16952388
13 J X Lin, X Jiang, J S Cao, F Fang, Q Feng (2018). Influences of phosphorous removal chemicals on EBPR system in wastewater treatment. Water Purification Technology, 37(12): 84–90 (in Chinese)
14 Y Liu, Z Liu, F Wang, Y Chen, P Kuschk, X Wang (2014). Regulation of aerobic granular sludge reformulation after granular sludge broken: Effect of poly aluminum chloride (PAC). Bioresource Technology, 158: 201–208
https://doi.org/10.1016/j.biortech.2014.02.002 pmid: 24607455
15 B Ma, G Chen, C Hu, Z Liu, H Liu, J Qu (2018). Speciation matching mechanisms between orthophosphate and aluminum species during advanced P removal process. Science of the Total Environment, 642(15): 1311–1319
https://doi.org/10.1016/j.scitotenv.2018.06.171 pmid: 30045511
16 W C Ma, L Zhao, H L Liu, Q L Liu, J Ma (2017). Improvement of sludge dewaterability with modified cinder via affecting EPS. Frontiers of Environmental Science and Engineering, 11(6): 19
17 M R Mehrnia, H Azami, M H Sarrafzadeh (2013). Fouling mitigation in membrane bioreactors using multivalent cations. Colloids and Surfaces. B, Biointerfaces, 109: 90–96
https://doi.org/10.1016/j.colsurfb.2013.03.009 pmid: 23624275
18 Y L Nie, X K Tian, Z X Zhou, Y Y Li (2017). Impact of food to microorganism ratio and alcohol ethoxylate dosage on methane production in treatment of low-strength wastewater by a submerged anaerobic membrane bioreactor. Frontiers of Environmental Science and Engineering, 11(6): 6
19 C Park, Y Fang, S N Murthy, J T Novak (2010). Effects of floc aluminum on activated sludge characteristics and removal of 17-a-ethinylestradiol in wastewater systems. Water Research, 44(5): 1335–1340
https://doi.org/10.1016/j.watres.2009.11.002 pmid: 19944440
20 S A Pino-Jelcic, S M Hong, J K Park (2006). Enhanced anaerobic biodegradability and inactivation of fecal coliforms and Salmonella spp. in wastewater sludge by using microwaves. Water Environment Research, 78(2): 209–216
https://doi.org/10.2175/106143005X90498 pmid: 16566529
21 X Ruan, L Li, J Liu (2013). Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances. Journal of Environmental Sciences-China, 25(5): 916–924
https://doi.org/10.1016/S1001-0742(12)60210-1 pmid: 24218821
22 Y Salama, M Chennaoui, A Sylla, M Mountadar, M Rihani, O Assobhei (2016). Characterization, structure, and function of extracellular polymeric substances (EPS) of microbial biofilm in biological wastewater treatment systems: A review. Desalination and Water Treatment, 57(35): 16220–16237
https://doi.org/10.1080/19443994.2015.1077739
23 G P Sheng, H Q Yu, X Y Li (2010). Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review. Biotechnology Advances, 28(6): 882–894
https://doi.org/10.1016/j.biotechadv.2010.08.001 pmid: 20705128
24 G P Sheng, H Q Yu, Z B Yue (2005). Production of extracellular polymeric substances from Rhodopseudomonas acidophila in the presence of toxic substances. Applied Microbiology and Biotechnology, 69(2): 216–222
https://doi.org/10.1007/s00253-005-1990-6 pmid: 15843928
25 A Sondhi, S Guha, C S Harendranath, A Singh (2010). Effect of aluminum (Al3+) on granulation in upflow anaerobic sludge blanket reactor treating low-strength synthetic wastewater. Water Environment Research, 82(8): 715–724
https://doi.org/10.2175/106143010X12609736966603 pmid: 20853750
26 D T Sponza (2003). Investigation of extracellular polymer substances (EPS) and physicochemical properties of different activated sludge flocs under steady-state conditions. Enzyme and Microbial Technology, 32(3–4): 375–385
https://doi.org/10.1016/S0141-0229(02)00309-5
27 M Sun, L Yan, L Zhang, L Song, J Guo, H Zhang (2019). New insights into the rapid formation of initial membrane fouling after in-situ cleaning in a membrane bioreactor. Process Biochemistry, 78: 108–113
https://doi.org/10.1016/j.procbio.2019.01.004
28 M Tian, F Zhao, X Shen, K Chu, J Wang, S Chen, Y Guo, H Liu (2015). The first metagenome of activated sludge from full-scale anaerobic/anoxic/oxic (A2O) nitrogen and phosphorus removal reactor using Illumina sequencing. Journal of Environmental Sciences-China, 35(1): 181–190
https://doi.org/10.1016/j.jes.2014.12.027 pmid: 26354707
29 C Wang, R He, Y Wu, M Lürling, H Cai, H L Jiang, X Liu (2017). Bioavailable phosphorus (P) reduction is less than mobile P immobilization in lake sediment for eutrophication control by inactivating agents. Water Research, 109(1): 196–206
https://doi.org/10.1016/j.watres.2016.11.045 pmid: 27888776
30 S Y Wang, Y L He, X Y Li, F X Jia, S Y Guo (2016). Optimization of extracellular polymeric substance extraction method of different sludge. Journal of Beijing University of Technology, 42(4): 569–576 (in Chinese)
31 B M Wilén, B Jin, P Lant (2003). The influence of key chemical constituents in activated sludge on surface and flocculating properties. Water Research, 37(9): 2127–2139
https://doi.org/10.1016/S0043-1354(02)00629-2 pmid: 12691899
32 T Yang, X K Hao, B Y Chen, Y X Chen, Y Zhang, K Yang (2018). Effects of Al3+ on dehydrogenase activity (DHA) and extracellular polymeric substances (EPS) of activated sludge in a sequencing batch biofilm reactor (SBBR). Acta Scientiae Circumstantiae, 38(4): 1453–1459 (in Chinese)
33 H F Zhang, M Sun, L F Song, J B Guo, L H Zhang (2019a). Fate of NaClO and membrane foulants during in-situ cleaning of membrane bioreactors: Combined effect on thermodynamic properties of sludge. Biochemical Engineering Journal, 147: 146–152
https://doi.org/10.1016/j.bej.2019.04.016
34 L H Zhang, M S Zhang, J B Guo, J Zheng, Z C Chen, H F Zhang (2019b). Effects of K+ salinity on the sludge activity and the microbial community structure of an A2O process. Chemosphere, 235: 805–813
https://doi.org/10.1016/j.chemosphere.2019.06.137 pmid: 31280049
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