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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (1) : 49-56     DOI: 10.1007/s11684-009-0012-y
Gene expression disparity in giant cell tumor of bone
Xiaohua PAN1,2, Shuhua YANG1(), Deming XIAO2, Yong DAI3, Lili REN3
1. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; 2. Department of Orthopaedics, Shenzhen People’s Hospital, The Second Medical College of Jinan University, Shenzhen 518020, China; 3. Clinical Research Center, Shenzhen People’s Hospital, The Second Hospital Medical College of Jinan University, Shenzhen 518020, China
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The aim of this paper was to study the differential gene expression of giant cell tumor of bone (GCTB) by gene chip technology. Total RNA of 8 fresh GCTB specimens (Jaffe I∶6 cases, II∶1 case, III∶1 case; Campanacci I∶6 cases, II∶1 case, III∶1 case; Enneking Staging G0T1-2M0: 5 cases, G1T1-2M0: 2 cases, G1T2M0: 1 case) and 4 normal bony callus specimens (the control group) were extracted and purified to get mRNA and then reverse transcribed to complementary DNA, respectively. Microarray screening with a set of 8064 human cDNA genes was conducted to analyze the difference among the samples and the control. The hybridization signals were scanned. The gene expression disparity between the GCTB samples and normal bony callus was significantly different (P<0.01), and the disparity of over 5-fold was found in 47 genes in the GCTB specimens, with 25 genes up-regulated and 22 down-regulated including the extracellular matrix and transforming-related genes, oncogene and its homolog genes, cytokine and its receptor genes. Specific gene spectrum associated with GCTB can be identified by cDNA microarray, which will be the foundation of progressive etiology elucidation, diagnosis and treatment of GCTB.

Keywords giant cell tumor of bone      gene      microarray, cDNA     
Corresponding Authors: YANG Shuhua,   
Issue Date: 05 March 2009
URL:     OR
gene tagGenBankUniGenegene namerelated function
MMP13N69322Hs.2936matrix metalloproteinase 13participates in collagen catabolism, albuminolysis; collagen protease and metalloendoprotease activity
DSPG3AA131238Hs.435680dermatan sulphate mucoprotein 3regulates glycosaminoglycan binding
DPTR48303Hs.80552skin pontine proteincell adhesion and protein binding
MMP12R92994Hs.645661matrix metalloproteinase 12endometrium adenocarcinoma oncogene
COL3A1T98612Hs.443625type III collagen α1participates in formation of connective tissue like skin and pneumoangiogram, mutation leads to type IV Ehlers-Danlos syndrome
MMP9T72581Hs.297413matrix metalloproteinase 9increases expression on abdominal aortic aneurysm anterior wall
COL1A2AA490172Hs.489142type I collagen α2encodes a chain of type I collagen, variation may lead to osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, atypical arachnodactyly
COL8A2AA780815Hs.353001type VIII collagen α2endothelial corneal dystrophy related
COL5A2AA461456Hs.445827type V collagen α2encodes an α chain of low abundance collagen fiber, related with type I, II Ehlers-Danlos syndrome
CTSSAA236164Hs.181301cathepsin Sfamily of C1 peptidase of lysosome cysteine proteinase
MMP2AA936799Hs.513617matrix metalloproteinase 2encodes the degradation of type IV collage enzyme
COL5A3AA994760Hs.235368type V collagen α3encodes an α chain of low abundance collagen fiber, related with Ehlers-Danlos syndrome
Tab.1  Table 1a Formation and transition with extracellular matrix related differentially up-regulated expression genes (12 genes)
gene tagGenBankUniGenegene namerelated function
MMP3 W51794Hs.645710matrix metalloproteinase 3collagen catabolism and calcium, and zinc deposition
COMPN94385Hs.1584cartilage oligomer matrix proteincalcific deposit and extracellular matrix structure composition, participates in beta -TGF signal pathway, closely related with skeleton development, activity of structure molecule
COL10A1AI828306Hs.520339collagen of type α1Xdown-regulation will cause epiphysis dyschondroplasia, maldevelopment or dysplasia
MMP7AA031514Hs.2256matrix metalloproteinase 7degrades proteoglycans, fibronectin, elastin, casein and participates in wound healing
COL1A2AA490172Hs.489142type I collagen α2encodes a chain of type I collagen, variation may lead to osteogenesis imperfecta, Ehlers-Danlos syndrome, Idiopathic osteoporosis, atypical arachnodactyly
COL6A1H99676Hs.474053type VI collagen α1mutation may lead to autosomal dominant disorder: Bethlem myopathy
COL18A1N81029Hs.517356type XVIII collagen α1related with spinal injury
MMP15AA443300Hs.80343matrix metalloproteinase 15cell surface matrix metalloproteinase
Tab.2  Table 1b Formation and transition with extracellular matrix related differentially down-regulated expression genes (8 genes)
gene tagGenBankUniGenegene namerelated function
PTTG1AA430032Hs.350966hypophysin tumor transforming gene 1participates in DNA metabolism, repair, replication and chromosomal disjunction. affects RNA polymerase 2 activity
ERBB3AA664212Hs.118681ornithoerythroblast leukemia virus tumorigenesis gene 3participates in the calcium signal pathway, transmembrane receptor protein tyrosine kinase (PTK) signal pathway, related with ATP, membrane protein phosphorylation, epidermal growth factor (EGF) receptor
MNDAN29376Hs.153837bone marrow nucleus differentiation antigenparticipates in cell defence reaction and cell transcription regulation
PIM2AA863383Hs.496096pim-2 oncogenerelated to carcinoma of prostate; participates in NF-kappaB activation pathway, regulates cell survival
RB1AA045192Hs.408528retinal glioblastoma factor 1related to retinal glioblastoma, osteosarcoma
FGRAA256172Hs.1422proto-oncogene tyrosine protein kinase FGRtyrosine protein kinase Src family member, negatively regulates cell migration and adherence
Tab.3  Table 2a Up-regulated expression of tumor related genes (6 genes)
gene tagGenBankUniGenegene namerelated function
PTGESAA436163Hs.146688prostaglandin E synthaseparticipates in prostaglandin metabolism and signal transduction
TYRO3W69497Hs.381282protein tyrosine kinase (PTK)participates in protein binding, receptor activity and signal transduction, etc
HYAL1AA464196Hs.75619hyaluronic acid glucosaminidase 1participates in glucosaminidase, hydrolysis enzymatic activity somato-regulation; significant efficacy on glycon synthesis and lysosome receptor activity
ELF3AA434373Hs.67928E74 like factor 3affects formation of biphasic differentiation synovial sarcoma epithelium structure
MPP2R60019Hs.514208membrane protein,palmitoylation 2affects cytoskeleton, regulates cell proliferation and signal pathway
ERBB2AA446928Hs.446352erythroblast leukemia virus tumorigenic gene homologue 2one of the encoding epidermal growth factor receptor family, breast carcinoma, ovarian cancer
Tab.4  Table 2b Up-regulated expression of tumor related genes (6 genes)
gene tagGenBankUniGenegene namerelated function
SCYA3AA677522Hs.514107small inducible cytokine A3regulates calcium ion dynamic equilibrium and cell movement, chemotactic factor, exocytosis, inflammatory response, signal transducer
TNFRSF11BAA194983Hs.81791tumor necrosis factor receptor family 11Bparticipates in apoptosis and skeletal development
SCYA18AA495985Hs.143961small inducible cytokine A18immunoregulation, inflammatory response related secretory protein; participates in inflammatory response
SCYA3L1R47893Hs.512683small inducible A3 like cytokine 1related with macrophage inflammatory protein
SCYB6AI889554Hs.164021small inducible cytokine subunit B6participates in neovascularization, affects tumor formation
SCYA4H62864Hs.655293small inducible cytokine A4macrophage inflammatory protein 1, lymphocyte activation
Tab.5  Table 3a Differential up-regulatied expression of cytokine and receptor family related genes (6 genes)
gene tagGenBankUniGenegene namerelated function
SCYD1R66139Hs. 6376small inducible cytokine D1chemotactic factor (C-X3 ligand 1), participates in immune response and calcium ion dependent cell-cell adhesion
SCYB14W72294Hs.483444small inducible cytokine B14chemotactic factor (C-X3 ligand 14)
IFNGR1H11482Hs.520414γ interferon 1participates in many kinds of immune response and signal transduction
TNFAIP2AA457114Hs.660676tumor necrosis factor, α-induced protein 2retinoic acid of target gene in acute promyelocytic leukemia (APL)
LILRB2H54023Hs.655652leukocyte immunoglobulin like receptor Bcontrols inflammatory reaction and cytotoxicity to centralize immunological reaction; recognition of MHC molecule HLA-G
Tab.6  Table 3b Differentially up-regulatied expression of cytokine and receptor family genes (5 genes)
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