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Frontiers of Agricultural Science and Engineering

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Front. Agr. Sci. Eng.    2020, Vol. 7 Issue (2) : 161-170    https://doi.org/10.15302/J-FASE-2019308
REVIEW
Base editing in pigs for precision breeding
Ruigao SONG1,2, Yu WANG3, Yanfang WANG3(), Jianguo ZHAO1,2()
1. State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2. Medical School, University of Chinese Academy of Sciences, Beijing 100049, China
3. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Abstract

Pigs are one of the most important domesticated animals and have great value in agriculture and biomedicine. Single nucleotide polymorphisms (SNPs) are a dominant type of genetic variation among individual pigs and contribute to the formation of traits. Precision single base substitution provides a strategy for accurate genetic improvement in pig production with the characterization of functional SNPs and genetic variants in pigs. Base editing has recently been developed as the latest gene-editing tool that can directly make changes in single nucleotides without introducing double-stranded DNA breaks (DSBs), providing a promising solution for precise genetic modification in large animals. This review summarizes gene-editing developments and highlights recent genetic dissection related to SNPs in major economic traits which may have the potential to be modified using SNP-editing applications. In addition, limitations and future directions of base editing in pig breeding are discussed.
Keywords base editing      genetic improvement      pigs      single nucleotide polymorphisms     
Corresponding Author(s): Yanfang WANG,Jianguo ZHAO   
Just Accepted Date: 02 March 2020   Online First Date: 27 March 2020    Issue Date: 28 April 2020
 Cite this article:   
Ruigao SONG,Yu WANG,Yanfang WANG, et al. Base editing in pigs for precision breeding[J]. Front. Agr. Sci. Eng. , 2020, 7(2): 161-170.
 URL:  
https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2019308
https://academic.hep.com.cn/fase/EN/Y2020/V7/I2/161
Functional SNP Gene Economic trait
c.265T>C[34] FASN Backfat fatty acid composition
c.2573T>C[53] MTTP Backfat fatty acid composition
g.G3072A[54] IGF2 Backfat thickness
c.555C>T[55] CTNNBL1 Backfat traits
c.892G>A[40] MC4R Fatness
c.205G>A[56] SLC39A7 Carcass traits
g.15G>A[57] CTSK Backfat thickness
g.233C>T[58] CRH Growth and body composition
c.131T>A[59] APOA2 Fatty acid composition
c.I199V[36] PRKAG3 Meat quality
c.T30N[36] PRKAG3 Meat quality
g.8227C>G[60] MUC4 Production traits
c.C2604T[61] PIK3C3 Production traits
g.A53G[62] IGFBP3 Litter size
g.35547A>G[63] ESR2 Sperm motility
g.158 A>C[64] PLCz Sperm concentration
g.358A>T[65] CD9 Sperm motility
g.C7462G[66] CXCL12 Pseudorabies virus disease resistance
c.12164+ 79G>A[66] BAT2 Immunological traits
c.86172+ 140C>T[66] Mx1 Immunological traits
g.G443A[67] TAP1 PRRSV resistance
c.933A>G[68] TLR3 PRRSV susceptibility
c.761A>G[69] IRF7 Health and immunity
g.2115T>C[70] LMP2 Haematological traits
g.1232C>G[70] LMP7 Haematological traits
c.C522T[71] BPI Disease resistance
c.A1060G[71] BPI Disease resistance
c.C1027A[72] TLR4 Salmonella shedding
c.8C>G[73] PSMB6 Immunological traits
c.144T>C[74] BCL10 Immunological traits
Tab.1  Putative functional SNPs for economically important traits in pigs
Fig.1  Base editing mediated gene pyramiding in pigs for breeding. The efficiency and accuracy in the editing of pig genome is greatly enhanced with the gene editing tools of HR to CRISPR and Base Editor and this makes it feasible to integrate merit alleles in one breed for improved production performance.
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