doi:10.1007/s11705-017-1637-z

Frontiers of Chemical Science and Engineering

2017, Vol. 11

Issue (1): 133-138 https://doi.org/10.1007/s11705-017-1637-z

本期目录

Production of rhamnolipids-producing enzymes of Pseudomonas in E. coli and structural characterization

Kata Kiss,Wei Ting Ng,Qingxin Li(

)

Institute of Chemical & Engineering Sciences, Agency for Science, Technology and Research, Jurong Island 627833, Singapore

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Abstract：

Rhamnolipids are a class of biosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in Pseudomonas aeruginosa. Four of the 5 proteins except RhlC were successfully over-expressed in E. coli and three of them (RhlA, RhlB and RhlI) were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins in vitro to favor the construction of super strains for rhamnolipids production.

Key words： rhamnolipids Pseudomonas RhlA RhlB RhlI protein folding

收稿日期: 2016-09-11 出版日期: 2017-03-17

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Corresponding Author(s): Qingxin Li

引用本文:

. [J]. Frontiers of Chemical Science and Engineering, 2017, 11(1): 133-138.
Kata Kiss, Wei Ting Ng, Qingxin Li. Production of rhamnolipids-producing enzymes of Pseudomonas in E. coli and structural characterization. Front. Chem. Sci. Eng., 2017, 11(1): 133-138.

链接本文:

https://academic.hep.com.cn/fcse/CN/10.1007/s11705-017-1637-z
https://academic.hep.com.cn/fcse/CN/Y2017/V11/I1/133

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