Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose beads

doi:10.1007/s11705-009-0213-6

Front Chem Eng Chin

2009, Vol. 3

Issue (3) : 229-234 https://doi.org/10.1007/s11705-009-0213-6

RESEARCH ARTICLE

Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose beads

Dongmei WANG^1,2, Guodong JIA¹, Liang XU¹, Xiaoyan DONG¹, Yan SUN¹(

)

1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 2. School of Pharmaceutical Sciences, Tianjin Medical University, Tianjin 300070, China

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Abstract

Anion-exchange superporous cellulose (DEAE-SC) and microporous cellulose (DEAE-MC) adsorbents were packed in an electrochromatographic column, and the effect of external electric field (eEF) on the dynamic adsorption was investigated. The column was designed to provide longitudinal, transverse or 2-dimensional (2D) eEF. It was found that the electro-kinetic effect caused by the introduction of an electric field played an important role in the dynamic adsorption of bovine serum albumin to the adsorbents. The dynamic binding capacity (DBC) in the presence of 2D eEF was higher than in the presence of a one-dimensional eEF. The effect of flow velocity on the DBC of the two adsorbents was also demonstrated. It was found that the effect of electric field on the DEAE-MC column was more remarkable than that on the DEAE-SC column at the same flow rate, whereas the DEAE-SC column showed higher DBC and adsorption efficiency (AE) than the DEAE-MC column. With increasing flow rate, the DEAE-SC column could still offer high DBC and AE in the presence of the 2D eEF. For example, a DBC of 21.4 mg/mL and an AE of 57.7% were obtained even at a flow rate as high as 900 cm/h. The results indicate that the 2D electrochromatography packed with the superporous cellulose adsorbent is promising for high-speed protein chromatography.

Keywords electrochromatography two-dimensional electric field dynamic binding capacity superporous cellulose bead protein

Corresponding Author(s): SUN Yan,Email:ysun@tju.edu.cn

Issue Date: 05 September 2009

Cite this article:

Dongmei WANG,Guodong JIA,Liang XU, et al. Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose beads[J]. Front Chem Eng Chin, 2009, 3(3): 229-234.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-009-0213-6
https://academic.hep.com.cn/fcse/EN/Y2009/V3/I3/229

Fig.1 Structure of the electrochromatographic column with 2D eEF
1. longitudinal electrode compartments; 2. transverse electrode compartments; 3. mini feeding compartments; 4. central gel compartment

Tab.1 Parameters of physical properties and adsorption equilibria

Tab.2 Comparison of between the different types of eEF on the column packed with DEAE-MC and DEAE-SC at the flow rate of 600 cm/h

Fig.2 Breakthrough curves of BSA on DEAE-SC (a) and DEAE-MC (b) with different types of eEF. L, longitudinal electric field; T, transverse electric field at 600 cm/h. The mobile phase was buffer A (3.9 mmol/L Tris, 47 mmol/L Gly, 5 mmol/L NaCl, pH 8.2). Sample solution was 2 mg/mL BSA prepared with buffer A

Tab.3 Comparison of DBC, AE and at different flow velocity in 2D eEF applied column packed with DEAE-MC and DEAE-SC

Fig.3 Breakthrough curves of BSA on DEAE-MC and DEAE-SC columns with 2D eEF at 600 cm/h. The mobile phase was buffer A (3.9 mmol/L Tris, 47 mmol/L Gly, 5 mmol/L NaCl, pH 8.2). Sample solution was 2 mg/mL BSA prepared with buffer A

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