A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

doi:10.1007/s11705-020-1950-9

Front. Chem. Sci. Eng.

2021, Vol. 15

Issue (2) : 410-420 https://doi.org/10.1007/s11705-020-1950-9

RESEARCH ARTICLE

A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Luanying He¹, Yulin Chang¹, Junhao Zhu¹, Ying Bi¹, Wenlin An¹, Yiyang Dong¹, Jia-Hui Liu^1,²(

), Shihui Wang¹(

)

¹. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
². Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

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Abstract

Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.

Keywords GO yeast polyelectrolyte cytoprotection nanomaterials

Corresponding Author(s): Jia-Hui Liu,Shihui Wang

Just Accepted Date: 28 June 2020 Online First Date: 31 July 2020 Issue Date: 10 March 2021

Cite this article:

Luanying He,Yulin Chang,Junhao Zhu, et al. A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation[J]. Front. Chem. Sci. Eng., 2021, 15(2): 410-420.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-020-1950-9
https://academic.hep.com.cn/fcse/EN/Y2021/V15/I2/410

Fig.1 Schematic illustration of the LbL encapsulation of yeast cells.

Fig.2 AFM images of GO 1 (a) and GO 2 (b).

Fig.3 Microscope images of mixtures of yeast and GO as a function of GO concentration. (a?c) Yeast and GO 1 mixtures with GO 1 concentrations of 2, 5, or 10 mg·mL^?¹, respectively; (d?f) Yeast and GO 2 mixtures with GO 2 concentrations of 2, 5, or 10 mg·mL^?¹, respectively; (g) yeast alone.

Fig.4 Microscope images of Y@GO encapsulated with different layers. (a) Yeast/PGA; (b) Yeast/PGA/PSS; (c) Yeast/PGA/PSS/PGA; (d) Yeast/PGA/PSS/PGA/GO1; (e) Yeast/PGA/PSS/PGA/GO1/PGA; (f) Yeast/PGA/PSS/PGA/GO1/PGA/PSS; (g) Yeast/PGA/PSS/PGA/GO2; (h) Yeast/PGA/PSS/PGA/GO2/PGA; (i) Yeast/PGA/PSS/PGA/GO2/PGA/PSS.

Fig.5 The CFU and zeta potentials of Y@GO encapsulated with different layers.

Fig.6 SEM images of (a) yeast, (b) Y@P, (c) Y@GO1, and (d) Y@GO2.

Fig.7 The growth curves of Y@GO, Y@P, and yeast cells.

Fig.8 The effects of pH on the proliferation and storage of Y@GO, Y@P, and yeast cells. (a) CFUs of Y@GO1, Y@GO2, Y@P, and yeast cells under different pH values; (b–e) CFUs of yeast, Y@P, Y@GO1, and Y@GO2 after the storage at different pH values and incubation times.

Fig.9 The effects of NaCl concentration on the proliferation and storage of Y@GO, Y@P, and yeast cells. (a) CFUs of Y@GO1, Y@GO2, Y@P, and yeast cells under different NaCl concentrations; (b?e) CFUs of yeast, Y@P, Y@GO1, and Y@GO2 after the storage at different NaCl concentrations and incubation times.

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