Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants

doi:10.1007/s11705-022-2277-5

Front. Chem. Sci. Eng.

2023, Vol. 17

Issue (7) : 867-879 https://doi.org/10.1007/s11705-022-2277-5

RESEARCH ARTICLE

Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants

Wei Zhang, Runtang Liu, Xu Yang, Binbin Nian, Yi Hu(

)

State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, China

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Abstract

Polydopamine-functionalized nanosilica was synthesized using an inexpensive and easily obtainable raw material, mild reaction conditions, and simple operation. Subsequently, a flexible spacer arm was introduced by using dialdehyde starch as a cross-linking agent to bind with laccase. A high loading amount (77.8 mg∙g^‒1) and activity retention (75.5%) could be achieved under the optimum immobilization conditions. Thermodynamic parameters showed that the immobilized laccase had a lower thermal deactivation rate constant and longer half-life. The enhancement of thermodynamic parameters indicated that the immobilized laccase had better thermal stability than free laccase. The residual activity of immobilized laccase remained at about 50.0% after 30 days, which was 4.0 times that of free laccase. Immobilized laccase demonstrated excellent removal of phenolic pollutants (2,4-dichlorophenol, bisphenol A, phenol, and 4-chlorophenol) and perfect reusability with 70% removal efficiency retention for 2,4-dichlorophenol after seven cycles. These results suggested that immobilized laccase possessed great reusability, improved thermal stability, and excellent storage stability. Organic–inorganic nanomaterials have a good application prospect for laccase immobilization, and the immobilized laccase of this work may provide a practical application for the removal of phenolic pollutants.

Keywords polydopamine pollutant removal thermodynamic phenolic pollutants immobilized laccase

Corresponding Author(s): Yi Hu

About author:

^* These authors contributed equally to this work.

Just Accepted Date: 02 December 2022 Online First Date: 03 April 2023 Issue Date: 05 July 2023

Cite this article:

Wei Zhang,Runtang Liu,Xu Yang, et al. Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants[J]. Front. Chem. Sci. Eng., 2023, 17(7): 867-879.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-022-2277-5
https://academic.hep.com.cn/fcse/EN/Y2023/V17/I7/867

Scheme1 Schematic illustration of the synthesis of PDA-functionalized nanoparticles, laccase immobilization, and application for pollutant removal.

Tab.1 Result of immobilization of laccase

Fig.1 (a) FTIR spectra and (b) TGA curves of carriers.

Fig.2 SEM and TEM images of (a–c) SiO₂ and (d–f) DAS/PDA/SiO₂.

Tab.2 Pore structure properties of supports

Fig.3 Nitrogen sorption isotherms of SiO₂, PDA/SiO₂, and DAS/PDA/SiO₂.

Fig.4 Effect of (a) pH and (b) temperature on the relative activity of free and immobilized laccase.

Tab.3 Kinetic parameters of immobilized laccase

Fig.5 Lineweaver–Burk plots of free and immobilized laccase.

Tab.4 Kinetic parameters of thermal deactivation of free and immobilized laccase

Fig.6 Dynamic curves of thermal deactivation of (a) free laccase and (b) immobilized laccase; (c) Arrhenius plots for the thermal deactivation of free laccase and immobilized laccase.

Tab.5 Thermodynamic parameters for thermal deactivation of free and immobilized laccase

Tab.6 Removals of phenolic compounds by different immobilized laccase catalysts

Fig.7 Degradation curves of (a) 2,4-DCP, (b) BPA, (c) PH, and (d) 4-CP.

Fig.8 (a) 2,4-DCP removal efficiencies of recycled immobilized laccase and (b) the residual activity of free laccase and immobilized laccase after storage.

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