Microcystin-LR biodegradation by <italic>Sphingopyxis</italic> sp. USTB-05

doi:10.1007/s11783-010-0261-7

Front Envir Sci Eng Chin

2011, Vol. 5

Issue (4) : 526-532 https://doi.org/10.1007/s11783-010-0261-7

RESEARCH ARTICLE

Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05

Chengbin XIAO¹, Hai YAN²(

), Junfeng WANG², Wei WEI², Jun NING¹, Gang PAN¹

1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. School of Chemical and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China

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Abstract

A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as Sphingopyxis sp. USTB-05, which was the first isolated MCs-biodegrading Sphingopyxis sp. in China. The average biodegradation rate of MC-LR by Sphingopyxis sp. USTB-05 was 28.8 mg·L^-1 per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30°C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg·L^-1 in water was biodegraded by Sphingopyxis sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg·L^-1 could be completely removed in 3 h by CE of Sphingopyxis sp. USTB-05 containing 350 mg·L^-1 protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.

Keywords microcystin-LR(MC-LR) biodegradation Sphingopyxis sp USTB-05 cell-free extract

Corresponding Author(s): YAN Hai,Email:haiyan@ustb.edu.cn

Issue Date: 05 December 2011

Cite this article:

Chengbin XIAO,Hai YAN,Junfeng WANG, et al. Microcystin-LR biodegradation by Sphingopyxis sp. USTB-05[J]. Front Envir Sci Eng Chin, 2011, 5(4): 526-532.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-010-0261-7
https://academic.hep.com.cn/fese/EN/Y2011/V5/I4/526

Fig.1 Colonies of strain USTB-05 grown on solid medium

Fig.2 Cell shape of strain USTB-05 under microscope (× 1000)

Fig.3 Phylogenetic tree of sp. USTB-05

Fig.4 Growth of sp. USTB-05 and biodegradation of MC-LR

Fig.5 Effects of pH (a) and temperature (b) on the biodegradation of MC-LR

Fig.6 Biodegradation of MC-LR remained in water and released from cyanobacterial cells collected from Lake Guishui by sp. USTB-05

Fig.7 Biodegradation of MC-LR by CE of sp. USTB-05 with different protein concentrations

Fig.8 HPLC profiles for biodegrading MC-LR by CE of sp. USTB-05 with time course. (a) 0 min; (b) 90 min; (c) 180 min; (d) 720 min

Fig.9 Absorbance profiles of MC-LR and its biodegradation products in the ultraviolet wavelength from 200 to 300 nm, respectively

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