Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus, <i>Aspergillus sydowii</i> BPOI

doi:10.1007/s11783-020-1292-3

Front. Environ. Sci. Eng.

2020, Vol. 14

Issue (6) : 113 https://doi.org/10.1007/s11783-020-1292-3

RESEARCH ARTICLE

Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus, Aspergillus sydowii BPOI

Paul Olusegun Bankole¹(

), Kirk Taylor Semple², Byong-Hun Jeon³, Sanjay Prabhu Govindwar³

¹. Department of Pure and Applied Botany, College of Biosciences, Federal University of Agriculture Abeokuta, Ogun State 234039, Nigeria
². Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK
³. Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, Republic of Korea

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Abstract

• A. sydowii strain bpo1 exhibited 99.8% anthracene degradation efficiency.

• Four unique metabolic products were obtained after anthracene degradation.

• Ligninolytic enzymes induction played vital roles in the removal of anthracene.

• Laccase played a crucial role in comparison with other enzymes induced.

The present study investigated the efficiency of Aspergillus sydowii strain bpo1 (GenBank Accession Number: MK373021) in the removal of anthracene (100 mg/L). Optimal degradation efficiency (98.7%) was observed at neutral pH, temperature (30℃), biomass weight (2 g) and salinity (0.2% w/v) within 72 h. The enzyme analyses revealed 131%, 107%, and 89% induction in laccase, lignin peroxidase, and manganese peroxidase respectively during anthracene degradation. Furthermore, the degradation efficiency (99.8%) and enzyme induction were significantly enhanced with the addition of 100 mg/L of citric acid and glucose to the culture. At varying anthracene concentrations (100–500 mg/L), the degradation rate constants (k₁) peaked with increasing concentration of anthracene while the half-life (t_1/2) decreases with increase in anthracene concentration. Goodness of fit (R² = 0.976 and 0.982) was observed when the experimental data were subjected to Langmuir and Temkin models respectively which affirmed the monolayer and heterogeneous nature exhibited by A. sydwoii cells during degradation. Four distinct metabolites; anthracene-1,8,9 (2H,8aH,9aH)-trione, 2,4a-dihydronaphthalene-1,5-dione, 1,3,3a,7a-tetrahydro-2-benzofuran-4,7-dione and 2-hydroxybenzoic acid was obtained through Gas Chromatography-Mass spectrometry (GC-MS). A. sydowii exhibited promising potentials in the removal of PAHs.

Keywords Aspergillus sydowii Anthracene Biodegradation Polycyclic aromatic hydrocarbons

Corresponding Author(s): Paul Olusegun Bankole

Issue Date: 13 November 2020

Cite this article:

Paul Olusegun Bankole,Kirk Taylor Semple,Byong-Hun Jeon, et al. Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus, Aspergillus sydowii BPOI[J]. Front. Environ. Sci. Eng., 2020, 14(6): 113.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1292-3
https://academic.hep.com.cn/fese/EN/Y2020/V14/I6/113

Fig.1 Phylogenetic typing through maximum-likelihood method of A. sydowii strain bpo1. The tree was inferred from the analysis of sequence data (ITS genes) with a scale bar of 0.020 indicating the genetic distance

Fig.2 Influence of (a) concentration, (b) temperature, (c) contact time, (d) salinity and (e) pH on degradation efficiencies and biomass. Graphs were plotted as mean±standard error of means

Fig.3 Influence of organic acids and glucose on (a) anthracene degradation efficiency, (b) induction of laccase, (c) induction of lignin peroxidase and (d) induction manganese peroxidase

Fig.4 Proposed metabolic pathway of anthracene degradation by A. sydowii

Tab.1 Comparison of anthracene biodegradation by A. sydowii strain bpo1 with reference to other fungi in similar conditions

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