Acceleration of the particulate organic matter hydrolysis by start-up stage recovery and its original microbial mechanism

doi:10.1007/s11783-020-1304-3

Front. Environ. Sci. Eng.

2021, Vol. 15

Issue (1) : 12 https://doi.org/10.1007/s11783-020-1304-3

RESEARCH ARTICLE

Acceleration of the particulate organic matter hydrolysis by start-up stage recovery and its original microbial mechanism

Yanqing Duan¹, Aijuan Zhou^1,²(

), Xiuping Yue^1,²(

), Zhichun Zhang¹, Yanjuan Gao¹, Yanhong Luo^1,³, Xiao Zhang¹

¹. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China
². Shanxi Engineer Research Institute of Sludge Disposition and Resources, Taiyuan University of Technology, Taiyuan 030024, China
³. School of Environment and Safety Engineering, North University of China, Taiyuan 030051, China

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Abstract

• Carbon availability was partially solved by POM recovery and fermentation.

• 12% carbon sources were regenerated by fermentation of the entrapped 35% TCOD.

• The unique microbial communities facilitated the efficient hydrolysis of the POM.

• Considerable economic benefits in aeration power and ECS dosage were anticipated.

To address the availability of carbon sources for denitrification, the accelerated hydrolysis of the most abundant but low-availability fraction of particulate organic matter (POM) was investigated. Mesh sieves with different pore sizes were used as primary pretreatment at the start-up-stage of the biological process to separate some POM from the liquid system. The changes in soluble carbohydrates and proteins were monitored to investigate the hydrolysis performance of the sieved POM, with waste activated sludge (WAS) as the control test. The results showed that an average of 35% POM could be entrapped before filtrate mat development. In addition, benefiting from the high polysaccharides concentration, as well as the high availability due to the relatively loose physical structure, a 23% hydrolysis efficiency of POM was obtained, in contrast to that of WAS (3.4%), with a hydrolysis constant of 0.39 h⁻¹. The prominent performance was also attributed to the unique microbial communities having been domesticated at a lower temperature, especially the cellulose-degrading bacteria Paraclostridium and psychrophile Psychrobacter, making up 6.94% and 2.56%, respectively. Furthermore, the potential benefits and application of improved POM hydrolysis by start-up stage recovery via mesh sieves combined with anaerobic fermentation were evaluated, including selective POM entrapment, alleviation of blockage and wear, and a reduction in aeration energy. By the proposed strategy, carbon availability for biological nutrient removal (BNR) processes is anticipated to be improved more economically than that can be achieved by primary clarifier elimination.

Keywords Particulate organic matter (POM) Hydrolysis Microbial community Mass balance

Corresponding Author(s): Aijuan Zhou,Xiuping Yue

Issue Date: 11 August 2020

Cite this article:

Yanqing Duan,Aijuan Zhou,Xiuping Yue, et al. Acceleration of the particulate organic matter hydrolysis by start-up stage recovery and its original microbial mechanism[J]. Front. Environ. Sci. Eng., 2021, 15(1): 12.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1304-3
https://academic.hep.com.cn/fese/EN/Y2021/V15/I1/12

Fig.1 Selective performance of particular organic matter (POM) by micro-sieving: (a) for POM concentration change with different pore size; (b) for particle size distribution before and after micro-sieving with a pore size of 0.131 mm (Note: error bars represent standard deviation).

Tab.1 Characteristics of R-POM and WAS

Fig.2 SEM images being magnified by 5000 times, (a) for WAS collected in the same plant; (b) and (c) for R-POM by sieves with pore sizes of 273 micron and 154 micron, respectively.

Fig.3 Time-course profiles of hydrolysis performance of R-POM and WAS fermentation: (a) for soluble polysaccharides; (b) for soluble proteins; (c) for fitting curve of SCOD release; (d) for hydrolytic enzyme activities (Note: error bars represent standard deviation).

Tab.2 Modeling statistics of Eq. (1) for the fitting SCOD-time profiles for R-POM and WAS

Fig.4 Taxonomic classifcation of pyrosequences from bacterial communities in R-POM and WAS at phylum (a), class (b) and genus (c) level.

Fig.5 Mass balance of the proposed R-POM entrapment and regeneration based on carbon fractions transformation and conversion (Unit: kg COD/d).

Tab.3 Sludge production and aeration consumption for the two processes

1	N K N Al-Shorgani, E Ali, M S Kalil, W M W Yusoff (2012). Bioconversion of butyric acid to butanol by Clostridium saccharoperbutylacetonicum N1–4 (ATCC 13564) in a limited nutrient medium. BioEnergy Research, 5(2): 287–293 https://doi.org/10.1007/s12155-011-9126-6
2	M Benneouala, Y Bareha, E Mengelle, M Bounouba, M Sperandio, Y Bessiere, E Paul (2017). Hydrolysis of particulate settleable solids (PSS) in activated sludge is determined by the bacteria initially adsorbed in the sewage. Water Research, 125(1): 400–409 https://doi.org/10.1016/j.watres.2017.08.058
3	T Bronn, R L Newcombe, S Fiepke (2008). Fine mesh, rotating belt sieve for primary treatment of municipal wastewater: Pilot project results. In: Proceedings of the Water Environment Federation 2008. Alexandria: IWA Publication, 1714–1718
4	H B Chen, D B Wang, X M Li, Q Yang, G M Zeng (2015). Enhancement of post-anoxic denitrification for biological nutrient removal: effect of different carbon sources. Environmental Science and Pollution Research International, 22(8): 5887–5894 https://doi.org/10.1007/s11356-014-3755-1
5	R Chen, L Guo, H Dang (2011). Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18. World Journal of Microbiology & Biotechnology, 27(2): 431–441 https://doi.org/10.1007/s11274-010-0475-7
6	D Crutchik, N Frison, A L Eusebi, F Fatone (2018). Biorefinery of cellulosic primary sludge towards targeted short chain fatty acids, phosphorus and methane recovery. Water Research, 136(1): 112-119 https://doi.org/10.1016/j.watres.2018.02.047
7	M I Daneshvar, M P Douglas, R S Weyant (2001). Cellular fatty acid composition of Lautropia mirabilis. Journal of Clinical Microbiology, 39(11): 4160–4162 https://doi.org/10.1128/JCM.39.11.4160-4162.2001
8	T Didem Okutman, K Ozlem,Güçlü İnsel Glinsel , Süleyman O Sleyman , O Derin, S Henri (2009). Biodegradability and denitrification potential of settleable chemical oxygen demand in domestic wastewater. Water Environment Research: A Research Publication of the Water Environment Federation, 81(7): 715–727 https://doi.org/10.2175/106143009X425942
9	Y Duan, A Zhou, K Wen, Z Liu, W Liu, A Wang, X Yue (2019). Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue. Frontiers of Environmental Science & Engineering, 13(1): 3–12 https://doi.org/10.1007/s11783-019-1086-7
10	G A Ekama, P L Dold, G V R Marais (1986). Procedures for determining influent COD fractions and the maximum specific growth rate of heterotrophs in activated sludge systems. Water Science and Technology, 18(6): 91–114 https://doi.org/10.2166/wst.1986.0062
11	A Z Gu, A Onnis-Hayden (2010). Protocol to evaluate alternative external carbon sources for denitrification at full-scale wastewater treatment plants. Water Environment Research Foundation, Report No. NUTR1R06b. New York: IWA Publication
12	J He, X Wang, X Yin, Q Li, X Li, Y Zhang, Y Deng (2018). Insights into biomethane production and microbial community succession during semi-continuous anaerobic digestion of waste cooking oil under different organic loading rates. AMB Express, 8(1): 92–102 https://doi.org/10.1186/s13568-018-0623-2
13	L He, F Y Ji, X L He (2013). Validation of accumulation models for inorganic suspended solids of different particle size in an activated sludge system. Bioresource Technology, 149:51–57 https://doi.org/10.1016/j.biortech.2013.09.042
14	M Henze, G Holm Kristensen, R Strube (1994). Rate-capacity characterization of wastewater for nutrient removal processes. Water Science and Technology, 29(7): 101–107 https://doi.org/10.2166/wst.1994.0318
15	Y Jiang, Y Shang, T Gong, Z Hu, K Yang, S Shao (2020). High concentration of Mn2+ has multiple influences on aerobic granular sludge for aniline wastewater treatment. Chemosphere, 240: 124945–124953 https://doi.org/10.1016/j.chemosphere.2019.124945
16	Z Jing, G Tao, Z Yu, H Jie, Z Liu-Qiang, L Wen-Jun, H Xing, L Shun-Peng (2012). Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas. International Journal of Systematic and Evolutionary Microbiology, 62(11): 2593–2597
17	A E Laloo, J Wei, D Wang, S Narayanasamy, I Vanwonterghem, D Waite, J Steen, A Kaysen, A Heintz-Buschart, Q Wang, B Schulz, A Nouwens, P Wilmes, P Hugenholtz, Z Yuan, P L Bond (2018). Mechanisms of persistence of the ammonia-oxidizing bacteria nitrosomonas to the biocide free nitrous acid. Environmental Science & Technology, 52(9): 5386–5397 https://doi.org/10.1021/acs.est.7b04273
18	X Lei, Y Li, G Wang, Y Chen, Q Lai, Z Chen, J Zhang, P Liao, H Zhu, W, Zheng T Zheng (2015). Phaeodactylibacter luteus sp. nov., isolated from the oleaginous microalga Picochlorum sp. International Journal of Systematic and Evolutionary Microbiology, 65(8): 2666–2670 https://doi.org/10.1099/ijs.0.000321
19	L Lin, L Wen, S Chen, X Yang, X Liu, C Wan (2015). Effect of alkaline treatment pattern on anaerobic fermentation of swine manure. Process Biochemistry, 50(11): 1710–1717 https://doi.org/10.1016/j.procbio.2015.08.011
20	J R Liu, R S Tanner, S Peter, W Norbert, C A Mckenzie, P H Janssen, E M Seviour, P A Lawson, T D Allen, R J Seviour (2002). Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low. International Journal of Systematic and Evolutionary Microbiology, 52(4): 1113–1127
21	Z Liu, A Zhou, H Liu, S Wang, W Liu, A Wang, X Yue (2020). Extracellular polymeric substance decomposition linked to hydrogen recovery from waste activated sludge: Role of peracetic acid and free nitrous acid co-pretreatment in a prefermentation-bioelectrolysis cascading system. Water Research, 176: 115724–115737 https://doi.org/10.1016/j.watres.2020.115724
22	D Mamais, D Jenkins, P Prrr (1993). A rapid physical-chemical method for the determination of readily biodegradable soluble COD in municipal wastewater. Water Research, 27(1): 195–197 https://doi.org/10.1016/0043-1354(93)90211-Y
23	C Martineau, C Villeneuve, F Mauffrey, R Villemur (2013). Hyphomicrobium nitrativorans sp. nov., isolated from the biofilm of a methanol-fed denitrification system treating seawater at the Montreal Biodome. International Journal of Systematic and Evolutionary Microbiology, 63(Pt 10): 3777–3781 https://doi.org/10.1099/ijs.0.048124-0
24	K D McMahon, M A Dojka, N R Pace, D Jenkins, J D Keasling (2002). Polyphosphate kinase from activated sludge performing enhanced biological phosphorus removal. Applied and Environmental Microbiology, 68(10): 4971–4978 https://doi.org/10.1128/AEM.68.10.4971-4978.2002
25	D Orhon (2015). Evolution of the activated sludge process: the first 50 years. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 90(4): 608–640 https://doi.org/10.1002/jctb.4565
26	I Pasztor, P Thury, J Pulai (2009). Chemical oxygen demand fractions of municipal wastewater for modeling of wastewater treatment. International Journal of Environmental Science and Technology, 6(1): 51–56 https://doi.org/10.1007/BF03326059
27	I R Ramsay, P C Pullammanappallil (2001). Protein degradation during anaerobic wastewater treatment: Derivation of stoichiometry. Biodegradation, 12(4): 247–256 https://doi.org/10.1023/A:1013116728817
28	C J Ruiken, G Breuer, E Klaversma, T Santiago, M C M van Loosdrecht (2013). Sieving wastewater: Cellulose recovery, economic and energy evaluation. Water Research, 47(1): 43–48 https://doi.org/10.1016/j.watres.2012.08.023
29	T S Sasi Jyothsna , L Tushar, C Sasikala, C V Ramana (2016). Paraclostridium benzoelyticum gen. nov. sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. Proposal of a new genus Paeniclostridium gen. nov. to accommodate Clostridium sordellii and Clostridium ghonii. International Journal of Systematic and Evolutionary Microbiology, 66(3): 1268–1274 https://doi.org/10.1099/ijsem.0.000874
30	Y Wang, Y Li, J Song, X Chen, C Zhang, Y Zhai, B Zhao, Z Ruan, B Zhao, H Wang, J Gerritsen (2015). Romboutsia sedimentorum sp. nov., isolated from alkaline-saline lake sediment and emended description of the genus Romboutsia. International Journal of Systematic and Evolutionary Microbiology, 65(4): 1193–1198 https://doi.org/10.1099/ijs.0.000079
31	B Wett (2006). Solved upscaling problems for implementing deammonification of rejection water. Water Science and Technology, 53(12): 121–128 https://doi.org/10.2166/wst.2006.413
32	E Xie, X Y Xu, G Y Luo (2013). Study on a novel reactor of sludge process reduction for domestic sewage treatment. Environmental Technology, 34(12): 1593–1599 https://doi.org/10.1080/09593330.2012.758670
33	G Yang, P Zhang, G Zhang, Y Wang, A Yang (2015). Degradation properties of protein and carbohydrate during sludge anaerobic digestion. Bioresource Technology, 192: 126–130 https://doi.org/10.1016/j.biortech.2015.05.076
34	K Zhang, L Song, X Dong (2010). Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen. International Journal of Systematic and Evolutionary Microbiology, 60(9): 2221–2225 https://doi.org/10.1099/ijs.0.011759-0
35	N Zuo, J He, X Ma, Y Peng, X Li (2016). Phosphorus removal performance and population structure of phosphorus-accumulating organisms in HA-A/A-MCO sludge reduction process. Bioengineered Bugs, 7(5): 327–333 https://doi.org/10.1080/21655979.2016.1197026

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