Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b

doi:10.1007/s11515-010-0840-9

Front Biol

2010, Vol. 5

Issue (5) : 439-444 https://doi.org/10.1007/s11515-010-0840-9

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Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b

Xiangpeng DAI^1,2, Xiaoyang ZHAO^1,2,3, Hai TANG^1,2, Jie HAO^1,2,3, Jean-Paul RENARD^2,4, Qi ZHOU^1,2, Alice JOUNEAU^2,4, Liu WANG^1,2(

)

1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 2. Sino-French Laboratory LABIOCEM, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.; 4. INRA, UMR 1198 Biologie du Développement et Reproduction, Jouy en Josas, France ENVA, Maison-Alfort, France

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Abstract

The epigenetic state of donor cells plays a vital role in the nuclear reprogramming and chromatin remodeling of cloned embryos. In this study we investigated the effect of DNA methylation state of donor cells on the development of mouse embryos reconstructed with embryonic stem (ES) cell nuclei. Our results confirmed that deletion of the DNA methyltransferase 3a (Dnmt3a) and DNA methyltransferase 3b (Dnmt3b) distinctly decreases the level of DNA methylation in ES cells. In contrast to wild type ES cells (J1), Dnmt3a-/-3b-/- (DKO) and Dnmt3b-/- (3bKO) donor cells significantly elevated the percentage of embryonic stem cell nuclear transfer (ECNT) morula, blastocysts and postimplantation embryos (P<0.05). However, the efficiency of establishment of NT-ES cell lines derived from DKO reconstructed blastocysts was not improved, and the expression pattern of OCT4 and CDX2 in cloned blastocysts and postimplantation embryos was not altered either. Our results suggest that the DNA methylation state of the donor nucleus is an important factor in regulation of the donor nuclear reprogramming.

Keywords DNA methylation nuclear transfer DNA methyltransferase 3a DNA methyltransferase 3b

Corresponding Author(s): WANG Liu,Email:wangliu@ioz.ac.cn

Issue Date: 01 October 2010

Cite this article:

Xiangpeng DAI,Xiaoyang ZHAO,Hai TANG, et al. Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b[J]. Front Biol, 2010, 5(5): 439-444.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-010-0840-9
https://academic.hep.com.cn/fib/EN/Y2010/V5/I5/439

Fig.1 DNA methylation intensity of different embryonic stem (ES) cells. A: The global DNA methylation level of J1 ES cell; B: The global DNA methylation level of DKO; C: The global DNA methylation level of 3aKO; D: The global DNA methylation level of 3bKO. Bars= 20 μm.

Tab.1 Pre-implantation development of ES-NT embryos

Tab.2 Post-implantation development of ECNT embryos

Tab.3 Establishment of NT-ES cells from DKO and J1 ES cell cloned blastocysts

Fig.2 Expression of Dmnt3a and Dnmt3b in NT-ES cells derived from J1 and DKO cloned blastocysts. A: Expression of Dnmt3a in ES cell derived from DKO cloned blastocyst; B: Expression of Dnmt3b in ES cell derived from DKO cloned blastocysts; C: Expression of Dnmt3a in ES cell derived from J1 cloned blastocyst; D: Expression of Dnmt3b in ES cell derived from J1 cloned blastocyst. Bars= 20 μm.

Fig.3 Expression of Oct4 (red) and CDX2 (green) in DKO, 3bKO, 3aKO, J1 cloned blastocyst and E6.5 embryo. A: DKO cloned blastocyst; B: 3bKO cloned blastocyst; C: 3aKO cloned blastocyst; D: J1 ES cell cloned blastocyst; E: DKO cloned E6.5 embryo; F: J1 cloned E6.5 embryo.

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