Single-cell genomics: An overview

doi:10.1007/s11515-013-1285-8

Front Biol

2013, Vol. 8

Issue (6) : 569-576 https://doi.org/10.1007/s11515-013-1285-8

REVIEW

Single-cell genomics: An overview

Qichao WANG¹, Xianmin ZHU^1,2, Yun FENG¹, Zhigang XUE¹, Guoping FAN^2,3,4(

)

1. Stem Cell Research Center, Department of Regenerative Medicine, Tongji University School of Medicine, Tongji University, Shanghai 200092, China; 2. Advanced Institute of Translational Medicine, Tongji University, Shanghai 200092, China; 3. School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; 4. Department of Human Genetics, UCLA, Los Angeles, CA 90095, USA

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Abstract

The newly developed next-generation sequencing platforms, in combination with genome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms.

Keywords single-cell genomics next-generation sequencing RNA-seq single-nucleotide variation copy-number variation DNA methylation

Corresponding Author(s): FAN Guoping,Email:Gfan@mednet.ucla.edu

Issue Date: 01 December 2013

Cite this article:

Qichao WANG,Xianmin ZHU,Yun FENG, et al. Single-cell genomics: An overview[J]. Front Biol, 2013, 8(6): 569-576.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-013-1285-8
https://academic.hep.com.cn/fib/EN/Y2013/V8/I6/569

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