<i>Zingiber officinale</i> extends <i>Drosophila melanogaster</i> life span in xenobiotic-induced oxidative stress conditions

doi:10.1007/s11515-018-1493-3

Front. Biol.

2018, Vol. 13

Issue (2) : 130-136 https://doi.org/10.1007/s11515-018-1493-3

RESEARCH ARTICLE

Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions

Volodymyr Padalko¹(

), Viktoriya Dzyuba², Olena Kozlova², Hanna Sheremet², Olena Protsenko¹

¹. School of Medicine, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61022, Ukraine
². Department of Membrane Biophysics, Research Institute of Biology, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61022, Ukraine

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Abstract

BACKGROUND: The possibility of dietary ginger to enhance oxidative stress resistance and to extend life span was studied on Drosophila melanogaster.

METHODS: Oxidative stress was induced by a reducing agent dithiothreitol. Experimental groups of male D. melanogaster were cultured on media containing: 1) no additive; 2) dithiothreitol, added into the nutritional mixture to the final concentration of 10 mM; 3) 25 mg of ginger powder g^–1 of the nutritional mixture; and 4) 10 mM of dithiothreitol and 25 mg of ginger powder g^–1 of the nutritional mixture. The number of alive fruit flies was inspected daily, and mean life span was determined for each experimental group.

RESULTS: The addition of dithiothreitol to D. melanogaster nutritional mixture was established to result in an increase in concentration of two markers of oxidative stress conditions (thiobarbituric acid reactive substances as products of lipid peroxidation and carbonylated proteins as products of protein oxidation) in fly tissues. It was followed by significant reduction of mean life span and maximum life span of the last 10% of flies. Plant preparation, being added simultaneously with dithiothreitol, significantly diminished the negative effects of this xenobiotic. In conditions of additional stress load induced by hydrogen peroxide or high temperature, survival of insects treated with dithiothreitol on the background of ginger powder was the highest.

CONCLUSIONS: Thus, the presented data give the evidence that ginger preparations can reduce oxidative stress outcomes and significantly increase the life expectancy of fruit flies in stress conditions.

Keywords ginger oxidative stress Drosophila melanogaster

Corresponding Author(s): Volodymyr Padalko

Online First Date: 17 May 2018 Issue Date: 28 May 2018

Cite this article:

Volodymyr Padalko,Viktoriya Dzyuba,Olena Kozlova, et al. Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions[J]. Front. Biol., 2018, 13(2): 130-136.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-018-1493-3
https://academic.hep.com.cn/fib/EN/Y2018/V13/I2/130

Tab.1 Thiobarbituric acid reactive substances (TBARS) and carbonylated protein (CP) content in control and experimental Drosophila melanogaster tissue crude extract.

Tab.2 Effect of DTT treatment on the survival time of the flies fed the standard diet or diet, containing ginger powder.

Fig.1 Survival curves of Drosophila melanogaster males fed basal diet (control), ginger powder (GP) containing diet and basal diet with dithiothreitol chronic exposure (DTT) or DTT containing diet with addition of ginger powder (DTT+ GP). The survival of flies on the basal diet and in the presence of the various additives was determined on samples of 20 males per vessel. The living flies were counted daily; the food was changed every two days. The initial number of flies was taken as 100%.

Tab.3 Effect of temperature stress (exposure to 37°C within one hour once a week for four weeks) on life span of Drosophila flies in the control group and DTT exposed groups with or without addition of ginger powder.

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