Comparative study of oxidative stress induced by sand flower and schistose nanosized layered double hydroxides in N2a cells

doi:10.1007/s11515-015-1344-4

Front. Biol.

2015, Vol. 10

Issue (3) : 279-286 https://doi.org/10.1007/s11515-015-1344-4

RESEARCH ARTICLE

Comparative study of oxidative stress induced by sand flower and schistose nanosized layered double hydroxides in N2a cells

Yu Lu¹,Biao Yan¹,Xudong Liu¹,Yuchao Zhang¹,Shibi Zeng²,Hao Hu²,Rong Xiang³,Yu Xu³,Ying Yu²,Xu Yang^1,^*(

)

1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life Science, Central China Normal University, Wuhan 430079, China
2. Institute of Nanoscience and Nanotechnology, Central China Normal University, Wuhan 430079, China
3. Department of Otolaryngology, Head & Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China

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Abstract

Magnesium–aluminum layered double hydroxide (Mg/Al–LDH) nanoparticles have strong potential application as drug delivery systems because of their low toxicity and suitable biocompatibility. However, few studies have described the morphological effects of these hydroxides on nerve cells. The present study compares the oxidative stress induced by different concentrations (i.e., 0, 50, 100, 200, 400, and 800 μg/mL) of sand flower and flake nano-Mg/Al–LDHs in mouse neuroblastoma cells (N2a) when these cells were exposed for 24 and 48 h. Cell viability was detected by MTT assay, and production of reactive oxygen species (ROS), glutathione (GSH), and malondialdehyde (MDA) were monitored to evaluate oxidative damage. Results suggested that sand flower nano-LDHs, at the appropriate concentrations (less than 200 μg/mL), especially those of about 100–200 nm in size, induce no harmful effects on N2a cells.

Keywords magnesium–aluminum layered double hydroxide mouse neuroblastoma cell oxidative stress reactive oxygen species

Corresponding Author(s): Xu Yang

Just Accepted Date: 06 March 2015 Online First Date: 17 April 2015 Issue Date: 23 June 2015

Cite this article:

Yuchao Zhang,Shibi Zeng,Hao Hu, et al. Comparative study of oxidative stress induced by sand flower and schistose nanosized layered double hydroxides in N2a cells[J]. Front. Biol., 2015, 10(3): 279-286.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-015-1344-4
https://academic.hep.com.cn/fib/EN/Y2015/V10/I3/279

Fig.1 Scanning electron microscopic images of synthesized nano-LDHs. (A) Sand flower nano-LDHs and (B) schistose nano-LDHs.

Fig.2 Morphological images of N2a cells exposed to nano-LDHs (stained with Hoechst 33258). The images are shown at 40× magnification. Row (A) shows N2a cells of the blank group. Row (B) shows images of N2a cells exposed to sand flower nano-LDHs at different concentrations (50, 100, 200, 400, and 800 μg/mL^-). Row (C) shows images of N2a cells exposed to schistose nano-LDHs at different concentrations (50, 100, 200, 400, and 800 μg/mL).

Fig.3 Effects of nano-LDHs on the viability of N2a cells (n = 5). * or #, p<0.05. ** or ##, p<0.01, compared with the control. &, p<0.05; &&, p<0.01, each sand flower nano-LDH group compared with the corresponding schistose nano-LDH group.

Fig.4 ROS levels in N2a cells (n = 5). (A) DCF fluorescence intensities induced by different concentrations of nano-LDH at 24 h. (B) DCF fluorescence intensities induced by different concentrations of nano-LDH at 48 h. * or #, p<0.05; ** or ##, p<0.01, compared with the control; &, p<0.05, each sand flower nano-LDH group compared with the corresponding schistose nano-LDH group.

Fig.5 GSH levels in N2a cells (n = 5). (A) GSH contents induced by different concentrations of nano-LDHs at 24 h. (B) GSH contents induced by different concentrations of nano-LDHs at 48 h. * or #, p<0.05; ** or ##, p<0.01, compared with the control.

Fig.6 MDA levels in N2a cells (n = 5). (A) MDA contents induced by different concentrations of nano-LDHs at 24 h. * or #, p<0.05, compared with the control. (B) MDA contents induced by different concentrations of nano-LDHs at 48 h. * or #, p<0.05; ** or ##, p<0.01, compared with the control. &&, p<0.01, each sand flower nano-LDH group compared with the corresponding schistose nano-LDH group.

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