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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

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Front Mater Sci    2011, Vol. 5 Issue (4) : 412-416    https://doi.org/10.1007/s11706-011-0147-z
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Cancer cell proliferation controlled by surface chemistry in its microenvironment
Xiao-Long YU1, Bin ZHANG2, Xiu-Mei WANG1, Ying WANG1, Lin QIAO1, Jin HE1, Juan WANG2, Shuang-Feng CHEN2, In-Seop LEE3, Fu-Zhai CUI1()
1. Institute of Regenerative and Biomimetic Materials, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China; 2. Liaocheng People’s Hospital, Liaocheng 252000, China; 3. Atomic-Scale Surface Science Research Center, Yonsei University, Seoul 120-749, Korea
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Abstract

Hepatoma cells (Hepg2s) as typical cancer cells cultured on hydroxyl (-OH) and methyl (-CH3) group surfaces were shown to exhibit different proliferation and morphological changes. Hepg2s cells on -OH surfaces grew much more rapidly than those on -CH3 surfaces. Hepg2s cells on -OH surfaces had the larger contact area and the more flattened morphology, while those on -CH3 surfaces exhibited the smaller contact area and the more rounded morphology. After 7 days of culture, the migration of Hepg2s cells into clusters on the -CH3 surfaces behaved significantly slower than that on the -OH surfaces. These chemically modified surfaces exhibited regulation of Hepg2s cells on proliferation, adhesion, and migration, providing a potential treatment of liver cancer.
Keywords chemical groups      cell proliferation      adhesion      migration      Hepg2     
Corresponding Author(s): CUI Fu-Zhai,Email:cuifz@mail.tsinghua.edu.cn   
Issue Date: 05 December 2011
 Cite this article:   
Xiao-Long YU,Bin ZHANG,Xiu-Mei WANG, et al. Cancer cell proliferation controlled by surface chemistry in its microenvironment[J]. Front Mater Sci, 2011, 5(4): 412-416.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-011-0147-z
https://academic.hep.com.cn/foms/EN/Y2011/V5/I4/412
Fig.1  The proliferation of Hepg2s cells cultured on different chemically modified substrates on days 3, 5, and 7 by the MTT assay.
Fig.2  SEM images of Hepg2s cells cultured on surfaces after 7 h with the chemical groups of -CH and -OH.
Fig.3  The morphology of Hepg2s cells cultured on chemically modified surfaces on days 1, 3, and 5 with a fluorescence confocal microscope: A1, A2, and A3 for -CH surface on days 1, 3, and 5, respectively; B1, B2, and B3 for -OH surface on days 1, 3, and 5, respectively.
Chemical groupContact angle/(° )
-OH64.25±1.5
-CH3103.00±5.0
Tab.1  Contact angle data by water as solvent (results are means±S.D. of three independent experiments)
Fig.4  XPS spectra of the Au surfaces with -OH groups (solid line) and -CH groups (dashed line). For -OH groups, the content of Au(4f) is 41.62% and that of S(2p) is 1.05%. For -CH groups, the content of Au(4f) is 43.20% and that of S(2p) is 2.05%.
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