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Frontiers of Materials Science

ISSN 2095-025X

ISSN 2095-0268(Online)

CN 11-5985/TB

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Front. Mater. Sci.    2015, Vol. 9 Issue (4) : 397-404    https://doi.org/10.1007/s11706-015-0316-6
RESEARCH ARTICLE
Evaluation on biocompatibility of biomedical polyurethanes with different hard segment contents
Dai-Wei MA1,2,Rong ZHU1,2,Yi-Yu WANG1,2,Zong-Rui ZHANG1,2,Xin-Yu WANG1,2,*()
1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China
2. Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China
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Abstract

In this paper, polyurethane (PU) materials with different contents of hard segment (20%, 25%, 30%) were prepared based on hexamethylene diisocyanate and polycarbonate diols by solution polymerization. The obtained polycarbonate-urethane (PCU) elastomers were characterized by very good hydrophobic property and excellent resistance to hydrolysis. Hemolysis, recalification time and platelet-rich plasma adhesion were used to evaluate the blood compatibility of the materials. L929 cells cultured with leach?liquor of these PU membranes were selected to perform the cytotoxicity experiments. The results indicate that the hemolysis rates of PU membranes are all less than 5%, which can meet the requirement of the national standards for biomaterials. However, compared with 20% and 30% groups, the recalification time of the sample containing 25% hard segment is longer, while the number of platelet adhesion is less. Additionally, cells cultured in the leach liquor of PU membranes with 25% hard segment proliferated relatively more thriving, meaning that this proportion of the material has the lowest cytotoxicity.
Keywords polyurethane (PU)      hydrolytic stability      blood compatibility      cytotoxicity     
Corresponding Author(s): Xin-Yu WANG   
Online First Date: 06 November 2015    Issue Date: 12 November 2015
 Cite this article:   
Dai-Wei MA,Rong ZHU,Yi-Yu WANG, et al. Evaluation on biocompatibility of biomedical polyurethanes with different hard segment contents[J]. Front. Mater. Sci., 2015, 9(4): 397-404.
 URL:  
https://academic.hep.com.cn/foms/EN/10.1007/s11706-015-0316-6
https://academic.hep.com.cn/foms/EN/Y2015/V9/I4/397
Fig.1  FTIR spectra of PCDL and prepared PU membranes.
Fig.2  The water contact angles of (a) PCU-1, (b) PCU-2, and (c) PCU-3.
Fig.3  Changes of mechanical properties of PCUs with different hard segment.
PCU specimen Weight change /%
5 d 10 d 15 d 20 d
PCU-1 +1.63 +1.62 +1.71 +1.98
PCU-2 +1.25 +1.25 +1.53 +1.66
PCU-3 +0.97 +0.99 +1.06 +1.12
Tab.1  Changes of weight observed for PCU specimens after immersion in buffer solution for different time
Sample Hemolysis ratio /% a)
PCU-1 0.8±0.04
PCU-2 0.2±0.07
PCU-3 0.4±0.06
Negative control 0±0.03
Positive control 1±0.02
Tab.2  Hemolysis activity of PU with different segments
Sample No. a) Recalcification time /s Average time /s
PCU-1 1 111 110
2 103
3 118
PCU-2 1 137 151
2 155
3 160
PCU-3 1 145 132
2 130
3 122
Tab.3  PRT of PU with different segments
Fig.4  SEM images of platelets adhering to PCU membranes with the content of (a) 20%, (b) 25% and (c) 30% hard segment.
Group OD550 CPR /% CTG
24 h 48 h 72 h 24 h 48 h 72 h 24 h 48 h 72 h
Blank 0.975±0.1 1.097±0.1 1.329±0.2
PCU-1 0.616±0.1 0.721±0.1 0.943±0.2 63.2 65.7 71.0 1 1 1
PCU-2 0.903±0.2 0.978±0.2 1.162±0.1 92.6 89.1 87.4 1 1 1
PCU-3 0.786±0.1 0.831±0.2 1.034±0.2 80.6 75.8 77.8 1 2 2
Negative 0.970±0.1 0.912±0.2 1.295±0.2 100 100 100 0 0 0
Positive 0.047±0.1 0.050±0.1 0.033±0.1 0 0 0 5 5 5
Tab.4  Cytotoxicity analysis of PU membranes
ACDanticoagulant citrate dextrose
ADPadenosine diphosphate
ATRattenuated total reflection
BDO1,4-butanediol
CPRcell proliferation rate
CTGcell toxicity grading
DBTDLdibutyltin dilaurate
DMFN,N-dimethyl formamide
DMSOdimethyl sulfoxide
EDTAethylene diamine tetraacetic acid
FBSfetal bovine serum
FIBfibrinogen
FTIRFourier transform infrared spectroscopy
MTT3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
ODoptical density
PBSphosphate buffered saline
PCDLpoly(1,6-hexanediol)carbonate diols
PCUpolycarbonate-urethane
PPPplatelet-poor plasma
PRPplatelet-rich plasma
PRTplasma recalcification time
PUpolyurethane
SEMscanning electron microscopy
THFtetrahydrofuran
Tab.1  
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