Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application

doi:10.1007/s11706-016-0353-9

Front. Mater. Sci.

2016, Vol. 10

Issue (4) : 358-366 https://doi.org/10.1007/s11706-016-0353-9

RESEARCH ARTICLE

Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application

Dong YAN,Zhong-Zheng ZHOU,Chang-Qing JIANG,Xiao-Jie CHENG,Ming KONG,Ya LIU,Chao FENG,Xi-Guang CHEN(

)

College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, China

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Abstract

A water absorption biomaterial, sodium carboxymethylation-functionalized chitosan fibers (Na-NOCC fibers) were prepared, applied for cutaneous wound repair, and characterized by FTIR and NMR. The water absorption of Na-NOCC fibers increased significantly with substitution degree rising, from 3.2 to 6.8 g/g, and higher than that of chitosan fibers (2.2 g/g) confirmed by swelling behavior. In the antibacterial action, the high degree of substitution of Na-NOCC fibers exhibited stronger antibacterial activities against E. coli (from 66.54% up to 88.86%). The inhibition of Na-NOCC fibers against S. aureus were above 90%, and more effective than E. coli. The cytotoxicity assay demonstrated that Na-NOCC2 fibers were no obvious cytotoxicity to mouse fibroblasts. Wound healing test and histological examination showed that significantly advanced granulation tissue and capillary formation in the healing-impaired wounds treated with Na-NOCC fibers, as compared to those treated with gauze, which demonstrated that Na-NOCC fibers could promote skin repair and might have great application for wound healing.

Keywords sodium carboxymethylation-functionalized chitosan fiber water absorption wound healing wound dressing

Corresponding Author(s): Xi-Guang CHEN

Online First Date: 18 September 2016 Issue Date: 24 November 2016

Cite this article:

Dong YAN,Zhong-Zheng ZHOU,Chang-Qing JIANG, et al. Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application[J]. Front. Mater. Sci., 2016, 10(4): 358-366.

URL:

https://academic.hep.com.cn/foms/EN/10.1007/s11706-016-0353-9
https://academic.hep.com.cn/foms/EN/Y2016/V10/I4/358

Fig.1 Schematic representation of the formation of Na-NOCC fibers.

Fig.2 FTIR spectra of CS fibers (a) and Na-NOCC fibers (b).

Fig.3 ¹H NMR spectra of (a) CS fibers and (b) Na-NOCC fibers.

Fig.4 Swelling ratio of Na-NOCC fibers with three DS and CS fibers. Bar graphs show *p<0.05 vs. CS fibers.

Fig.5 Photomicrographs of (a) dry CS fibers, (b) wet CS fibers, (c) dry Na-NOCC fibers, and (d) wet Na-NOCC fibers.

Fig.6 Antibacterial activities of Na-NOCC fibers with three DS and CS fibers. Bar graphs show *p<0.05 vs. CS fibers.

Fig.7 Cytotoxicity test of Na-NOCC2 fibers by mouse fibroblast at 24, 48 and 72 h. Bar graphs show *p<0.05.

Fig.8 The wound healing area at different times. Bar graphs show *p<0.05 vs. CS fibers.

Fig.9 Wound healing analysis in animal model: (a) Gauze; (b) Na-NOCC2 fibers.

Fig.10 Histomorphological photograph of the skin biopsies at 6 and 12 d of treatments (I: Inflammatory cells; Epi: Epidermis): (a) Gauze; (b) Na-NOCC2 fibers.

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