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Transcriptome assembly strategies for precision medicine |
Lu Wang1, Lipi Acharya2, Changxin Bai1, Dongxiao Zhu1( ) |
1. Department of Computer Science, Wayne State University, Detroit, MI 48202, USA 2. Dow AgroSciences, Indianapolis, IN 46268, USA |
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Abstract Background: Precision medicine approach holds great promise to tailored diagnosis, treatment and prevention. Individuals can be vastly different in their genomic information and genetic mechanisms hence having unique transcriptomic signatures. The development of precision medicine has demanded moving beyond DNA sequencing (DNA-Seq) to much more pointed RNA-sequencing (RNA-Seq) [Cell, 2017, 168: 584–599]. Results: Here we conduct a brief survey on the recent methodology development of transcriptome assembly approach using RNA-Seq. Conclusions: Since transcriptomes in human disease are highly complex, dynamic and diverse, transcriptome assembly is playing an increasingly important role in precision medicine research to dissect the molecular mechanisms of the human diseases.
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Author Summary Precision medicine is an emerging area in healthcare that aims to provide personalized diagnosis, treatment, and prevention by taking into account an individual’s genetic information, environment, and lifestyle. Transcriptome assembly approaches enable an in-depth understanding of the cellular mechanisms and cellular processes and thus plays a key role in the precision medicine. In this review article, we survey a number of recently developed transcriptome assembly strategies including de novo transcriptome assembly, reference-based transcriptome assembly, and de novo and reference-based combined strategies. |
Keywords
precision medicine
transcriptome assembly
RNA-Seq
de novo
De Bruijn
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Corresponding Author(s):
Dongxiao Zhu
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Just Accepted Date: 08 June 2017
Online First Date: 31 October 2017
Issue Date: 04 December 2017
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