Expression pattern and regulation of head-to-head genes <i>Vps36</i> and <i>Ckap2</i> during chicken follicle development

doi:10.15302/J-FASE-2014013

Front. Agr. Sci. Eng.

2014, Vol. 1

Issue (2) : 130-136 https://doi.org/10.15302/J-FASE-2014013

RESEARCH ARTICLE

Expression pattern and regulation of head-to-head genes Vps36 and Ckap2 during chicken follicle development

Xinxing CUI¹,Chunhong YANG¹,Li KANG¹,Guiyu ZHU²,Qingqing WEI¹,Yunliang JIANG^1,^*(

)

1. Laboratory of Animal Molecular Genetics, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
2. Department of Biology Science and Technology, Taishan University, Taian 271021, China

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Abstract

Vacuolar protein sorting 36 (VPS36), a protein primarily known for its role in the Endosomal Sorting Complex Required for Transport pathway, has recently been shown to be linked to chicken reproduction. Previous research showed that Vps36 is significantly downregulated in sexually mature chicken ovaries compared to immature ones. In this study, using real-time quantitative RT-PCR, we investigated the expression pattern of Vps36 and its head-to-head gene Ckap2 mRNA in chicken follicles. Small white follicles were found to have significantly higher expression of Vps36 and Ckap2 mRNA than any other sized follicles (P<0.05). The expression of Vps36 and Ckap2 mRNA were detected in both granulosa and theca layers of pre-ovulatory follicles, the expression of Ckap2 in theca layers was slightly higher than in granulosa cells. Treatment of small yellow follicles with follicle-stimulating hormone and estradiol resulted in a marked decrease of both Vps36 and Ckap2 mRNA (P<0.05); however, progesterone, transforming growth factor-β 1 and luteinizing hormone induced no significant changes in Vps36 and Ckap2 mRNA expression in these follicles. These results indicate that the head-to-head genes of Vps36 and Ckap2 exhibit similar expression in chicken follicles and are involved in chicken follicle development.

Keywords chicken Vps36 Ckap2 mRNA follicle

Corresponding Author(s): Yunliang JIANG

Online First Date: 22 September 2014 Issue Date: 10 October 2014

Cite this article:

Xinxing CUI,Chunhong YANG,Li KANG, et al. Expression pattern and regulation of head-to-head genes Vps36 and Ckap2 during chicken follicle development[J]. Front. Agr. Sci. Eng. , 2014, 1(2): 130-136.

URL:

https://academic.hep.com.cn/fase/EN/10.15302/J-FASE-2014013
https://academic.hep.com.cn/fase/EN/Y2014/V1/I2/130

Tab.1 Oligonucleotide primer sequences for real-time quantitative PCR

Fig.1 Vps36 (a) and Ckap2 (b) mRNA abundance in different sized follicles as measured by real-time quantitative RCR. The different letters above each bar indicate significant difference at P < 0.05. Data are means ± standard error of the mean (n = 4). SWF (small white follicles, 2-4 mm); SYF (small yellow follicles, 4-8 mm); F5 (12-14 mm); F3 (22-24 mm); F2 (the second largest follicle); F1 (the largest follicle).

Fig.2 Real-time quantitative PCR analysis of Vps36 and Ckap2 gene expression in granulosa and thecal layer cells of pre-ovulatory (F1, F3 and F6) follicles. Values marked with different letters differ significantly (P < 0.05). Data are means ± SEM (n = 5).

Fig.3 Effect of FSH (a) and LH (b) on mRNA expression of Vps36 and Ckap2 in chicken small yellow follicles. Values marked with different letters differ significantly (P < 0.05). Data are means ± SEM (n = 4).

Fig.4 Effects of E2 (a) and P4 (b) on mRNA expression of Vps36 and Ckap2 in chicken small yellow follicles. Values marked with different letters differ significantly (P < 0.05). Data are means ± SEM (n = 4).

Fig.5 Effect of TGFβ1 on mRNA expression of Vps36 and Ckap2 in chicken small yellow follicles. Values marked with different letters differ significantly (P < 0.05). Data are means ± SEM (n = 4).

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