Current technologies to identify protein kinase substrates in high throughput

doi:10.1007/s11515-013-1257-z

Front Biol

2013, Vol. 8

Issue (2) : 216-227 https://doi.org/10.1007/s11515-013-1257-z

REVIEW

Current technologies to identify protein kinase substrates in high throughput

Liang XUE¹, W. Andy TAO^1,2,3,4(

)

1. Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA; 2. Department of Medicinal Chemistry & Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA; 3. Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA; 4. Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA

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Abstract

Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.

Keywords phosphorylation kinase substrate in vitro kinase assay high throughput screening mass spectrometry phosphoproteomics

Corresponding Author(s): TAO W. Andy,Email:watao@purdue.edu

Issue Date: 01 April 2013

Cite this article:

Liang XUE,W. Andy TAO. Current technologies to identify protein kinase substrates in high throughput[J]. Front Biol, 2013, 8(2): 216-227.

URL:

https://academic.hep.com.cn/fib/EN/10.1007/s11515-013-1257-z
https://academic.hep.com.cn/fib/EN/Y2013/V8/I2/216

Fig.1 High throughput strategies to identify kinase substrates. ASKA, analog sensitive kinase allele; MS, mass spectrometry; AP-MS, affinity purification-mass spectrometry.

Fig.2 Kinase assay based on protein array or peptide array. Protein/peptide collections are spotted on the microarray, followed by the incubation with a purified active kinase under the reaction condition. Phosphorylation is detected by various methods.

Fig.3 Workflow for phage display-based substrate screening. From a large population of phages, phosphorylated phage can be either identified by autoradiography or phosphorylation-specific antibodies.

Fig.4 Application of the classical yeast-two-hybrid approach for the kinase substrate identification. Each candidate is paired with the kinase of interest. Putative substrates showing positive interaction must be examined by alternative experimental strategies.

Fig.5 Brief illustration of analog sensitive kinase allele strategy (ASKA). (A)The bulky-ATP analog can be only up taken by engineered kinase. (B) Proposed application of ASKA to identify direct kinase substrates both and .

Fig.6 Flow chart for the Kinase Assay Linked with Phosphoproteomics (KALIP) approach to identify direct kinase substrates.

Tab.1 Comparisons of kinase substrate screening techniques (See text for more details)

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